Sequences from confirmation amplicon


Confirmation sequences for line 155A07 and hit At5g01490 are shown.
Line ID 155A07
Locus At5g01490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:196102
Primers used for PCR RU33 - 8474, show primer details
   
Sequence Name 31-K023369-022-155-A07C-8409
GenBank Accession LN499987 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K023369-022-155-A07C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATTACAAACATATATT
TTGATATATATAATGATATAATGATATAAACATATATAAGAAAACATATATTTTGATTTT
CTTCTTAAACAGGCAAATTGCTTTCCATACCGGTCCAACAGGTGATCATTTAGATTTCGT
TGACTTTATATTTGTTGCCATGTGAATCGAACCGGGTTATTCGCAGCACAAAACCGAATT
CTTAAATTAAATCAACGCATTATTTTTGTGGTTTAATCTAGGTGTCTTTTATAGTGCATT
GATCTTTGATAACTTGACATGCAAGCTATATACATGAAGGAAATTGGATTTTTGATTCTG
TGAAAGTGTTTGCATTTTCTCTCACTTATGAAAATTAATGAAAATAATTGAATGAATGAA
TGAAGTTGGAGGATTAATGAACGCCACATGTGGAAACGCAACGGAGATGATAATAGCGAT
TCTAGCGGTTGGGCAAAGGAAGATGAGAATAGTGAAACTCTCACTTCTTGGCTCTATTCT
CTCCAAT
   
Sequence Name 32-K023369-022-155-A07C-RU33
GenBank Accession LN500413 [GenBank]
Sequencing primer gene specific primer, RU33: 5'-AAGACGAAGAGAAGATTGGAGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K023369-022-155-A07C-RU33
CAGCCAAGAAGTGAGAGTTTCACTATTCTCATCTTCCTTTGCCCAACCGCTAGAATCGCT
ATTATCATCTCCGTTGCGTTTCCACATGTGGCGTTCATTAATCCTCCAACTTCATTCATT
CATTCAATTATTTTCATTAATTTTCATAAGTGAGAGAAAATGCAAACACTTTCACAGAAT
CAAAAATCCAATTTCCTTCATGTATATAGCTTGCATGTCAAGTTATCAAAGATCAATGCA
CTATAAAAGACACCTAGATTAAACCACAAAAATAATGCGTTGATTTAATTTAAGAATTCG
GTTTTGTGCTGCGAATAACCCGGTTCGATTCACATGGCAACAAATATAAAGTCAACGAAA
TCTAAATGATCACCTGTTGGACCGGTATGGAAAGCAATTTGCCTGTTTAAGAAGAAAATC
AAAATATATGTTTTCTTATATATGTTTATATCATTATATCATTATATATATCAAAATATA
TGTTTGTAATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATNTCGACGTGTCTAC
Primers used for PCR FN35 - 3144, show primer details
   
Sequence Name 52-K032733-022-155-A07C-FN35
GenBank Accession LN507719 [GenBank]
Sequencing primer gene specific primer, FN35: 5'-AGAGTTCGTTGGGAGTTTATGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K032733-022-155-A07C-FN35
GAGTTCTTCTCGGCACCAAATTGTTCATCCTCTTCCCGGCCGTTCCTCTCGCCGTCGTCG
CTCACCGCTATGACTGTCCACGTGTAAGTTCCAACAAATATATCTTTATTTTATCGGAAT
AATAAACCAAACATTCGTCTTAAACCATATGTTATGTTAATTGTTATTGCGTGGTGTAAC
AAAAAAACAGGCGTGGGTCTTTGCGTTGAGCTTGCTGGGATTGACACCTTTGGCCGAACG
TATCAGTTTTCTCACCGAGTAATTTATTCTTCTTTGTTTTCTTCTTTTACCATTTATTTG
CTTTCTATCATTATATGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACC
CTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGT
GGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAG
ACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGG
   
Sequence Name 20-K032733-022-155-A07C-3144
GenBank Accession LN495108 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K032733-022-155-A07C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACATATAATGATAGAAAGCAAATA
AATGGTAAAAGAAGAAAACAAAGAAGAATAAATTACTCGGTGAGAAAACTGATACGTTCG
GCCAAAGGTGTCAATCCCAGCAAGCTCAACGCAAAGACCCACGCCTGTTTTTTTGTTACA
CCACGCAATAACAATTAACATAACATATGGTTTAAGACGAATGTTTGGTTTATTATTCCG
ATAAAATAAAGATATATTTGTTGGAACTTACACGTGGACAGTCATAGCGGTGAGCGACGA
CGGCGAGAGGAACGGCCGGGAAGAGGATGAACAATTTGGTGCCGAGAAGAACTTCTTGAA
GATTCGTCAAGATTCGTCTCATAAACTCCCANCCGAACT

Last Updated on 06.12.2018 16:11