Sequences from confirmation amplicon


Confirmation sequences for line 157C08 and hit At4g37940 are shown.
Line ID 157C08
Locus At4g37940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17836468
Primers used for PCR SZ01 - 8474, show primer details
   
Sequence Name 29-K015965-022-157-C08C-8409
GenBank Accession LN499071 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K015965-022-157-C08C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGGCGATACCGATGATTTTCTTGCAAAGCATG
CAGTTCTTGTCTTAGAACAGCAGCTTCTCTCTGCCAAAACTGTAACACACAACACAAATA
ACTCATCAAACTTTGAACAGCTCAGTAAACCAAAATCTCATTTCAAAAATCAAACATCAT
GCTAAAGCATCAATCAATATATAATCACATATCTTTAGAGTTGTCGCTATATCTATATGT
ATACCTTGACTTCTGATGCGGGGTTCAATAGTTGTTGTTGCTCGATCTTGCTCTTGTTGT
ATCTATCAATAACCGACTTCATGCTATTCAAAATATTTAATATTAGTATACCTTGTTCAT
GGTATATAATTCACTCATATCATATCATCTCTTAGAGTAAATTACGTAAAAGATATTAAT
TTTCGATCGTGAAGTTTTTCCAAGTACTCCATTAAAAAGCTTTCATACGTAAAGAAAAAA
CTCATAGCCTAGAAATTTGTGCAATATAGACTTTCCTGCATTGGCAACAT
   
Sequence Name 30-K015965-022-157-C08C-SZ01
GenBank Accession LN499507 [GenBank]
Sequencing primer gene specific primer, SZ01: 5'-GTGTGTAAATAATGTTGCCAATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K015965-022-157-C08C-sz01
GGAAAGTCTATATTGCACAAATTTCTAGGCTATGAGTTTTTTCTTTACGTATGAAAGCTT
TTTAATGGAGTACTTGGAAAAACTTCACGATCGAAAATTAATATCTTTTACGTAATTTAC
TCTAAGAGATGATATGATATGAGTGAATTATATACCATGAACAAGGTATACTAATATTAA
ATATTTTGAATAGCATGAAGTCGGTTATTGATAGATACAACAAGAGCAAGATCGAGCAAC
AACAACTATTGAACCCCGCATCAGAAGTCAAGGTATACATATAGATATAGCGACAACTCT
AAAGATATGTGATTATATATTGATTGATGCTTTAGCATGATGTTTGATTTTTGAAATGAG
ATTTTGGTTTACTGAGCTGTTCAAAGTTTGATGAGTTATTTGTGTTGTGTGTTACAGTTT
TGGCAGAGAGAAGCTGCTGTTCTAAGACAAGAACTGCATGCTTTGCAAGAAAATCATCGG
TATCGCCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTAC
Primers used for PCR NF02 - 8474, show primer details
   
Sequence Name 23-K113214-0022-157-C08C-8409
GenBank Accession LN496632 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K113214-0022-157-C08C-8409
TCGGACATGAGCCATTTACAATTGGCGATACCGATGATTTTCTTGCAAGCATGCAGTTCT
TGTCTTAGAACAGCAGCTTCTCTCTGCCAAAACTGTAACACACAACACAAATAACTCATC
AAACTTTGAACAGCTCAGTAAACCAAAATCTCATTTCAAAAATCAAACATCATGCTAAAG
CATCAATCAATATATAATCACATATCTTTAGAGTTGTCGCTATATCTATATGTATACCTT
GACTTCTGATGCGGGGTTCAATAGTTGTTGTTGCTCGATCTTGCTCTTGTTGTATCTATC
AATAACCGACTTCATGCTATTCAAAATATTTAATATTAGTATACCTTGTTCATGGTATAT
AATTCACTCATATCATATCATCTCTTAGAGTAAATTACGTAAAAGATATTAATTTTCGAT
CGTGAAGTTTTTCCAAGTACTCCATTAAAAAGCTTTCATACGTAAAGAAAAAACTCATAG
CCTAGAAATTTGTGCAATATAGACTTTCCTGCATTGGCAACATTATTTACACAC
   
Sequence Name 23-K113215-0022-157-C08C-NF02
GenBank Accession LN496633 [GenBank]
Sequencing primer gene specific primer, NF02: 5'-GTGTGTAAATAATGTTGCCAATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K113215-0022-157-C08C-NF02
ATTTCTGGCTTGAGTTTTTTCTTTCGTATGAAAGCTTTTTAATGGAGTACTTGGAAAAAC
TTCACGATCGAAAATTAATATCTTTTACGTAATTTACTCTAAGAGATGATATGATATGAG
TGAATTATATACCATGAACAAGGTATACTAATATTAAATATTTTGAATAGCATGAAGTCG
GTTATTGATAGATACAACAAGAGCAAGATCGAGCAACAACAACTATTGAACCCCGCATCA
GAAGTCAAGGTATACATATAGATATAGCGACAACTCTAAAGATATGTGATTATATATTGA
TTGATGCTTTAGCATGATGTTTGATTTTTGAAATGAGATTTTGGTTTACTGAGCTGTTCA
AAGTTTGATGAGTTATTTGTGTTGTGTGTTACAGTTTTGGCAGAGAGAAGCTGCTGTTCT
AAGACAAGAACTGCATGCTTTGCAAGAAAATCATCGGTATCGCCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11