Sequences from confirmation amplicon


Confirmation sequences for line 157G04 and hit At1g06490 are shown.
Line ID 157G04
Locus At1g06490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1987037
Primers used for PCR VL33 - 8474, show primer details
   
Sequence Name 57-K022563-022-157-G04C-8409
GenBank Accession LN509285 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K022563-022-157-G04C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATGATGGATCAATTAAATTTTAG
ATCGTTCTCTACCTGCAAAGATATCTTCGCTTAAGTTTATTATCTTTGAAGCCTTGCTAA
TGCCTCCTCTTGTGATGTGGAAGATTCTATCAAATATATCAGGATGGCCATAATGGAAAC
GCACCCTGTAAAAGAATCGTGGGTAAGTTGCCAAAGACCAAAGCTAGTCTTCTTTCCATA
ACAAAAAGATTCTAACTATAATATAGTCTATTAGGTTCAATGATGCGGGGACTGCTACTT
TTAAATAAGATTTGAAGCTACAAAGGAAGGATTGGAAGGATCTTTTCCCTAGTCACAAGA
TATGAAAATACCTCAGAGGATTTGCCAGAACACGTTGGCCAATGGTTACAAAACTTGTTT
CT
   
Sequence Name 58-K022563-022-157-G04C-VL33
GenBank Accession LN509581 [GenBank]
Sequencing primer gene specific primer, VL33: 5'-TGTCGAATCAAGAAACAAGTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K022563-022-157-G04C-VL33
CATTGGCCAACGTGTTCTGGCAAATCCTCTGAGGTATTTTCATATCTTGTGACTAGGGAA
AAGATCCTTCCAATCCTTCCTTTGTAGCTTCAAATCTTATTTAAAAGTAGCAGTCCCCGC
ATCATTGAACCTAATAGACTATATTATAGTTAGAATCTTTTTGTTATGGAAAGAAGACTA
GCTTTGGTCTTTGGCAACTTACCCACGATTCTTTTACAGGGTGCGTTTCCATTATGGCCA
TCCTGATATATTTGATAGAATCTTCCACATCACAAGAGGAGGCATTAGCAAGGCTTCAAA
GATAATAAACTTAAGCGAAGATATCTTTGCAGGTAGAGAACGATCTAAAATTTAATTGAT
CCATCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTACATT
Primers used for PCR VL34 - 8474, show primer details
   
Sequence Name 01-K022564-022-157-G04C-8409
GenBank Accession LN486401 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K022564-022-157-G04C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCTTGACAAGTTTTTGTTTT
CTAATTATTTTGGTGTATTTGTGATGGCTGATCATGGTAACATCCAGGGTACAATTCAAC
TCTTCGTGGGGGTTACGTCACACATCACGAGTATATTCAAGCAGGGAAAGGTCGTGATGT
AGGGATGAATCAGATTTCGTTTTTTGAAGCCAAAGTTGCGAATGGTAATGGAGAACAAAC
ACTAAGTCGTGACGTTTACCGACTTGGACGCCGGTTTGATTTTTACAGGATGCTTTCATT
CTACTTCACCACAGTTGGTTTCTATTTCAGTAGTATGGTATGCCTCATTTCTCATTCAAA
TAGTTATTTCCTCTTGTAGCTCTGCCCT
   
Sequence Name 02-K022564-022-157-G04C-VL34
GenBank Accession LN486856 [GenBank]
Sequencing primer gene specific primer, VL34: 5'-ACCAAAGTATAGGGCAGAGCTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K022564-022-157-G04C-VL34
AAACTATTTGAATGAGAAATGAGGCATACCATACTACTGAAATAGAAACCAACTGTGGTG
AAGTAGAATGAAAGCATCCTGTAAAAATCAAACCGGCGTCCAAGTCGGTAAACGTCACGA
CTTAGTGTTTGTTCTCCATTACCATTCGCAACTTTGGCTTCAAAAAACGAAATCTGATTC
ATCCCTACATCACGACCTTTCCCTGCTTGAATATACTCGTGATGTGTGACGTAACCCCCA
CGAAGAGTTGAATTGTACCCTGGATGTTACCATGATCAGCCATCACAAATACACCAAAAT
AATTAGAAAACAAAAACTTGTCAAGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11