Sequences from confirmation amplicon


Confirmation sequences for line 158D11 and hit At4g12020 are shown.
Line ID 158D11
Locus At4g12020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7202736
Primers used for PCR IS03 - 8474, show primer details
   
Sequence Name 65-K118874-0022-158-D11C-IS03
GenBank Accession LN511503 [GenBank]
Sequencing primer gene specific primer, IS03: 5'-AAGAAGAGCAATAGAAGGGAAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K118874-0022-158-D11C-IS03
CCCTATTAAAGAAAATAGGAAAGACCTATCTAAAATAACCAAGTCTACCTGATCAAAAGG
CACCGAGAAAAAAATTGTAGATGAATCTTAAACCTGATCATGATGAAATAGGTATCGGGT
TTACTACATTATATACATACCTCAACCTTGTCAAGAAAATCGATAGGGAAATCATCCTCG
GGCAGAATGGTTATTTGGTACTCCTTTGACAAGTAGGTATTAAAACCCAAAAGTAAGTCG
TCATGGCCCTTGAACAAATCTTTTAATCTGGCTATGACGCCACTAGTGTCAACTCTACAT
CCAACATGAGTCACCACAAAAAATTAATATATTTCCTGCTGTAAGAAGAGAGACATAATG
GAAAGATAGAAGAGAGTACCCCTGATGTTTACAGTCATTCAAGACTTCAAGGAAAGTGTC
ATATTTCTCACTGTCTTCAAACTTGTCCTTCACAGCTTTGAGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
   
Sequence Name 25-K118873-0022-158-D11C-8409
GenBank Accession LN497678 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K118873-0022-158-D11C-8409
ATTTACATTGAATATATCTCAAAGCTGTGAAGGACAAGTTTGAAGACAGTGAGAAATATG
ACACTTTCCTTGAAGTCTTGAATGACTGTAAACATCAGGGGTACTCTCTTCTATCTTTCC
ATTATGTCTCTCTTCTTACAGCAGGAAATATATTAATTTTTTGTGGTGACTCATGTTGGA
TGTAGAGTTGACACTAGTGGCGTCATAGCCAGATTAAAAGATTTGTTCAAGGGCCATGAC
GACTTACTTTTGGGTTTTAATACCTACTTGTCAAAGGAGTACCAAATAACCATTCTGCCC
GAGGATGATTTCCCTATCGATTTTCTTGACAAGGTTGAGGTATGTATATAATGTAGTAAA
CCCGATACCTATTTCATCATGATCAGGTTTAAGATTCATCTACAATTTTTTTCTCGGTGC
CTTTTGATCAGGTAGACTTGGTTATTTTAGATAGGTCTTTCCTATTTTCTTTTAATAGGG
GGATTTAGTTTTCAACTCAAGGTAACTAGCCGGTTTCCCTT

Last Updated on 06.12.2018 16:11