Sequences from confirmation amplicon


Confirmation sequences for line 161D12 and hit At4g38380 are shown.
Line ID 161D12
Locus At4g38380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17974291
Primers used for PCR BM11 - 8474, show primer details
   
Sequence Name 25-K015838-022-161-D12C-8409
GenBank Accession LN497217 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K015838-022-161-D12C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATGCAGTCTCCATTAGCA
GCGTCAAAGGATCAATAGCTTGGCCAGCAATGGCTGGAAGAGAGAGCATCACGAGTTCTC
GTTTGATATCGACAGGACGTGCTACACCAGTGTGAACCCCATTAACCTCCCTAGCAAGAA
CGGAGACCAAAGCATTACATAGGCAAGAAACGCAAAGCAACATGGCCAAAGAGTAACAAA
GGGATAACGCAACACCTCAATAGCCTTACGGGAGTTTGTCCAGTGAAGAGCAAGAGTCAT
CTTCTTCCCCCAACTTGACGACACCGCAATCAGGGTTAGTCTGGCTCTTGCGGCGAGTAA
CAAAAGGGAACATGGGGTTTGGATTGGTGTGAAGGGAGCATTTTTGGGGGGCAGAGACAT
TATTGATCCGAGATAGAGGTAGAAAAGTCCCTTGTTGGATTTTGGGTTTGGCAAAGCTAG
AGTTCCTCCTATTCGCCAAGGGCAGCCCACCACCCACTACTACTCTACTGGACTCCATCA
CACACAGACACTGCAAAAACGAAACTTTACTAGTTCGTCTATATATAATTAGAACCAAAA
GAAAGAAA
   
Sequence Name 26-K015838-022-161-D12C-BM11
GenBank Accession LN497690 [GenBank]
Sequencing primer gene specific primer, BM11: 5'-TCGAAGCTTTCTTTCTTTCTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K015838-022-161-D12C-bm11
CTAATTATATATAGACGAACTAGTAAAGTTTCGTTTTTGCAGTGTCTGTGTGTGATGGAG
TCCAGTAGAGTAGTAGTGGGTGGTGGGCTGCCCTTGGCGAATAGGAGGAACTCTAGCTTT
GCCAAACCCAAAATCCAACAAGGGACTTTTCTACCTCTATCTCGGATCAATAATGTCTCT
GCCCCCCAAAAATGCTCCCTTCACACCAATCCAAACCCCATGTTCCCTTTTGTTACTCGC
CGCAAGAGCCAGACTAACCCTGATTGCGGTGTCGTCAAGTTGGGGGAAGAAGATGACTCT
TGCTCTTCACTGGACAAACTCCCGTAAGGCTATTGAGGTGTTGCGTTATCCCTTTGTTAC
TCTTTGGCCATGTTGCTTTGCGTTTCTTGCCTATGTAATGCTTTGGTCTCCGTTCTTGCT
AGGGAGGTTAATGGGGTTCACACTGGTGTAGCACGTCCTGTCGATATCAAACGAGAACTC
GTGATGCTCTCTCTTCCAGCCATTGCTGGCCAAGCTATTGATCCTTTGACGCTGCTAATG
GAGACTGCATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAA
ATT

Last Updated on 06.12.2018 16:11