Sequences from confirmation amplicon


Confirmation sequences for line 166H07 and hit At5g19330 are shown.
Line ID 166H07
Locus At5g19330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6510000
Primers used for PCR EK02 - 8474, show primer details
   
Sequence Name 25-K019977-022-166-H07C-EK02
GenBank Accession LN497242 [GenBank]
Sequencing primer gene specific primer, EK02: 5'-GGTTGTGGAAGATGGGTATTCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K019977-022-166-H07C-EK02
TGTTTCTAAATCTGTTTTCATTAGGGGGAACTTTTAAGTAGTGAGTTATATTAAATAATA
AAACCGATGTGTCTTTTGATTTCTAGCATACTCTTTCTTGCAGGATGCCCACAATCAAGC
TGGTATTGCCCATAGTGGTGGTTTAGGACCTTTATTGAAGCTTCTCGATTCAAGAAATGG
ATCATTGCAACACAATGCTGCATTTGCTCTTTATGGCCTTGCCGATAATGAGGTGTGCTG
TATGCTACTTTCGGTTTATTCATTATGCAGAATTTGATGGTAAACGTCGGTAAATGGGTT
ATTGATCCATTTTTCTTCCCCTAGGATAATGTGTCAGATTTTATCAGGGTGGGAGGTATC
CAAAAGCTACAGGATGGAGAGTTTATTGTTCAAGTATGTATGGCGTTGCCACTCTTCCTC
TACTGAAATCAGGATACTGAAGTTTTGGAGAAAAAGTAAATGACAATCTGATCCCCGTAT
ATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCNGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATTTCGACGTGTCTAC
   
Sequence Name 29-K019977-022-166-H07C-8409
GenBank Accession LN499095 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K019977-022-166-H07C-8409
ATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATATACGGGGATC
AGATTGTCATTTACTTTTTCTCCAAAACTTCAGTATCCTGATTTCAGTAGAGGAAGAGTG
GCAACGCCATACATACTTGAACAATAAACTCTCCATCCTGTAGCTTTTGGATACCTCCCA
CCCTGATAAAATCTGACACATTATCCTAGGGGAAGAAAAATGGATCAATAACCCATTTAC
CGACGTTTACCATCAAATTCTGCATAATGAATAAACCGAAAGTAGCATACAGCACACCTC
ATTATCGGCAAGGCCATAAAGAGCAAATGCAGCATTGTGTTGCAATGATCCATTTCTTGA
ATCGAGAAGCTTCAATAAAGGTCCTAAACCACCACTATGGGCAATACCAGCTTGATTGTG
GGCATCCTGCAAGAAAGAGTATGCTAGAAATCAAAAGACACATCGGTTTTATTATTTAAT
ATAACTCACTACTTAAAAGTTCCCCCTAATGAAAACAGATTTAGAAACACAATAAGAATA
CCCAT

Last Updated on 06.12.2018 16:11