Sequences from confirmation amplicon


Confirmation sequences for line 167D08 and hit At5g15980 are shown.
Line ID 167D08
Locus At5g15980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5214897
Primers used for PCR NE46 - 8474, show primer details
   
Sequence Name 07-K102498-0022-167-D08C-8409
GenBank Accession LN489219 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K102498-0022-167-D08C-8409
ATTTACAATTGAATATATGCACTCAAAATGTAGATTTCTCCATTTTTGTATGTGCTATCC
CTTTCAGTTGATTTTTCGTTACTTGTGCACTCAAAAGCTTGTATGCATCTCTTACTTGAT
TCTTGTTGCAATAAGCAAGAACCAACTTCTCAAATGAATAATCAGCATAGGAGACTCCCG
TGTTTCCAACCATTTTCTCGAAACACACTAAAGCTTCATCAAGATTCCCGGAATCACAAT
ACCCTTCAACAAGAGACGCCATTGCCTTGTCATCTAGATTGTTTCCGGATGATTCCATAA
AGTCTACAAATTCATCAGCTTCGTCCTTCTTTCCCTTACGACTAAGACTTGATGCAATCA
TGCTCTGCATATCACCACTAGGAACATAGCCTCCTCTCTTCATTTCTTTCAACAGCTC
   
Sequence Name 07-K102499-0022-167-D08C-NE46
GenBank Accession LN489220 [GenBank]
Sequencing primer gene specific primer, NE46: 5'-TGAGCTGTTGAAAGAAATGAAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K102499-0022-167-D08C-NE46
ATGATTGCATCAGTCTTAGTCGTAAGGGAAAGAAGGACGAAGCTGATGAATTTGTAGACT
TTATGGAATCATCCGGAAACAATCTAGATGACAAGGCAATGGCGTCTCTTGTTGAAGGGT
ATTGTGATTCCGGGAATCTTGATGAAGCTTTAGTGTGTTTCGAGAAAATGGTTGGAAACA
CGGGAGTCTCCTATGCTGATTATTCATTTGAGAAGTTGGTTCTTGCTTATTGCAACAAGA
ATCAAGTAAGAGATGCATACAAGCTTTTGAGTGCACAAGTAACGAAAAATCAACTGAAAG
GGATAGCACATACAAAAATGGAGAAATCTACATTTTGAGTGCATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11