Sequences from confirmation amplicon


Confirmation sequences for line 168B12 and hit At4g39330 are shown.
Line ID 168B12
Locus At4g39330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18292311
Primers used for PCR ZZ26 - 8474, show primer details
   
Sequence Name 35-K020244-022-168-B12C-8409
GenBank Accession LN501650 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K020244-022-168-B12C-8409
AGATTTCCCGGACATGAAGCCATTTACAAAACCAAGTTAAAGTCACTCTCTTTACTTTCT
GAACTGAACCGGATTGGACCAAGTTTAAAATCACTCTCGTTACCTTCATTTTCTGAGGAT
CAGTTGTGACAAGAAACGAATCAGCACCAAGATGATTAATGGCTTCCTCTGCTTTCGTAG
AAGAAGAACTAATGACAGTAACTTTCAAACCAAAAGCTTTACCAATCTTAACAGCAACAT
GACCAAGCCCACCAAGTCCAGCAACCCCTAAATGCTTCCCTGCCTCAGTCATACCATAAT
ACTTCATTGGACTATACACAGTGATTCCAGCACACAGCAACGGCGCACCCGAATCGCTCG
GTAAATTCTCCGGAAACCGCAAAACAAACCGTTGATCAACCACAATGTTCTCCGAATAGC
CACCGTAATTCTTGGTTCCATCGGATCCAATCGCATTGTATGTGAAAGACATTTGAGGAC
AGTAGTTTTCAAGATCTTGGTCACAAGATTCGCAAGATTGGCACGAGCCACTGATCACTC
CTACTCCGACACGATCTCCTTCTTTGAATTTAGTCACGTTCTTACCAACTTTTGTAGCGA
TCCCAACGATTTCATGCCTAATTAATATACAAGGTTTTGTTATATAATAGAAAACTTGTT
AAATACGATATCTCTGTTTGAAATTTTGTTTCGAGATTACCCTGGAACTACTGGGTAATA
CGAGTATCCCCAGTCGTTTTTGATGGTGTGTAAATC
   
Sequence Name 36-K020244-022-168-B12C-ZZ26
GenBank Accession LN502048 [GenBank]
Sequencing primer gene specific primer, ZZ26: 5'-CTGTTCGTTTCTCTGTTGTCTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K020244-022-168-B12C-ZZ26
GATTGGTCTAATTTTTACTCTGCAGAGACAATGGTGAAAATGATGTGACAGTGAAGATCT
TGTTCTGTGGAGTTTGCCACACTGATTTACACACCATCAAAAACGACTGGGGATACTCGT
ATTACCCAGTAGTTCCAGGGTAATCTCGAAACAAAATTTCAAACAGAGATATCGTATTTA
ACAAGTTTTCTATTATATAACAAAACCTTGTATATTAATTAGGCATGAAATCGTTGGGAT
CGCTACAAAAGTTGGTAAGAACGTGACTAAATTCAAAGAAGGAGATCGTGTCGGAGTAGG
AGTGATCAGTGGCTCGTGCCAATCTTGCGAATCTTGTGACCAAGATCTTGAAAACTACTG
TCCTCAAATGTCTTTCACATACAATGCGATTGGATCCGATGGAACCAAGAATTACGGTGG
CTATTCGGAGAACATTGTGGTTGATCAACGGTTTGTTTTGCGGTTTCCGGAGAATTTACC
GAGCGATTCGGGTGCGCCGTTGCTGTGTGCTGGAATCACTGTGTATAGTCCAATGAAGTA
TTATGGTATGACTGAGGCAGGGAAGCATTTAGGGGTTGCTGGACTTGGTGGGCTTGGTCA
TGTTGCTGTTAAGATTGGTAAAGCTTTTGGTTTGAAAGTTACTGTCATTAGTTCTTCTTC
TACGAAAGCAGAGGAAGCCATTAATCATCTTGGTGCTGATTCGTTTCTTGTCACAACTGA
TCCTCAGAAAATGAAGGTAACGAGAGTGATTTTAAACTTGGTCCAATCGGTTCAGTTCAG
AAAGTAAAGAGAGTGACTTTTACTTGGTTTTG

Last Updated on 06.12.2018 16:11