Sequences from confirmation amplicon


Confirmation sequences for line 168C11 and hit At3g60190 are shown.
Line ID 168C11
Locus At3g60190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22247250
Primers used for PCR BM69 - 8474, show primer details
   
Sequence Name 05-K104415-0022-168-C11C-BM69
GenBank Accession LN488340 [GenBank]
Sequencing primer gene specific primer, BM69: 5'-ACTGAGGTGAATAGGAACTGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K104415-0022-168-C11C-BM69
GTCCATCCTGAATCTCCCTGCGAACCAAAGCTGAAACATCGAAGAATTTTCAAGCAAAAA
TTCAATGAACACTTTCAACTAAAACACACTTGAAGCAGAACAAACATACATTGATATACA
TAACATAGATAGACATAAATGTTAAGGTACATACCAAAATCTGTGAATTGCTTCTTGGGA
AGATGAAGGAACTCTGCATACTCCTCTGTTCCATCATCAGTCTTATGAAGCTGCAACACT
AAAGGCCGGGAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 21-K104416-0022-168-C11C-8409
GenBank Accession LN495646 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K104416-0022-168-C11C-8409
CCATTTACATTGAATATTTCCCGGCCTTTAGTGTTGCAGCTTCATAAGACTGATGATGGA
ACAGAGGAGTATGCAGAGTTCCTTCATCTTCCCAAGAAGCAATTCACAGATTTTGGTATG
TACCTTAACATTTATGTCTATCTATGTTATGTATATCAATGTATGTTTGTTCTGCTTCAA
GTGTGTTTTAGTTGAAAGTGTTCATTGAATTTTTGCTTGAAAATTCTTCGATGTTTCAGC
TTTGGTTCGCAGGGAGATTCAGGATGAGACTGATAGAATCACAGGGAAAAACAAACAGAT
ATCTCCAGTTCCTATTCACCTC

Last Updated on 06.12.2018 16:11