Sequences from confirmation amplicon


Confirmation sequences for line 170E12 and hit At3g15690 are shown.
Line ID 170E12
Locus At3g15690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5317206
Primers used for PCR LB17 - 8474, show primer details
   
Sequence Name 35-K033707-022-170-E12C-8409
GenBank Accession LN501731 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K033707-022-170-E12C-8409
CCATTTTCAATTTGAATGCTTTCGTAGTTTTCTGCTCTCATAGAATCATATTCATCGAAA
GGTTGTAACTTTGGGGATTCTGTTTATTGAGTGATAGGAAAACTCAGAAAGGGACTTAAC
TTTGAACAATTAGGTTGATTTTGGTATAAATTAGAGATCTAAAGTTGAAGAATTTGTCTT
CAGTATCTGTTTCAATGGAGATGAGATTCAAGTTACTTCATTTGATATTGAATTGCCAAG
CTAATCTAATTGATGAGTTTGGCAGTGATAAGTTAATTTCATAATTTGTATCTCTTAATA
TGAATTACTCGACAACATTACTTAATCTTTCACTGTTGAGTATACGTGGAGATCGGTTAA
CGTGAGTTTATTCTAAGACATTATATTTTGAATTACTTAAAACTTTCTGGAGCTATCTTG
GATTGAGTGTATAAGATTTGCTTATGCTCAATTTTAAAAAGTGAGGGATCATATTGAAGA
TAAGTGCTTATTTAGTCTTTCTTTTTGACTCTGTCTTGTTTGGNCTGAT
   
Sequence Name 35-K033707-022-170-E12C-LB17
GenBank Accession LN501732 [GenBank]
Sequencing primer gene specific primer, LB17: 5'-AAATCAGCCAAAACAAGACAGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K033707-022-170-E12C-LB17
ATAGCCTTATCTTCATATGATCCCTCACTTTTTAAAATTGAGCATAAGCAAATCTTATAC
ACTCAATCCAAGATAGCTCCAGAAAGTTTTAAGTAATTCAAAATATAATGTCTTAGAATA
AACTCACGTTAACCGATCTCCACGTATACTCAACAGTGAAAGATTAAGTAATGTTGTCGA
GTAATTCATATTAAGAGATACAAATTATGAAATTAACTTATCACTGCCAAACTCATCAAT
TAGATTAGCTTGGCAATTCAATATCAAATGAAGTAACTTGAATCTCATCTCCATTGAAAC
AGATACTGAAGACAAATTCTTCAACTTTAGATCTCTAATTTATACCAAAATCAACCTAAT
TGTTCAAAGTTAAGTCCCTTTCTGAGTTTTCCTATCACTCAATAAACAGAATCCCCAAAG
TTACAACCTTTCGATGAATATGATTCTATGAGAGCAGAAAACTACGAAAGCATTCAAATT
TGAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
ATT

Last Updated on 06.12.2018 16:11