Sequences from confirmation amplicon


Confirmation sequences for line 171D05 and hit At1g54270 are shown.
Line ID 171D05
Locus At1g54270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20261710
Primers used for PCR AK31 - 8474, show primer details
   
Sequence Name 12-K105704-0022-171-D05C-AK31
GenBank Accession LN491577 [GenBank]
Sequencing primer gene specific primer, AK31: 5'-GACGGGTCAAAAATCAAATAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K105704-0022-171-D05C-AK31
CTTCTTCTTCACAGCAAATCAGCCACGTTTGAGGGAAGCTCCTCAACAACCACATTGTAG
AATTTCTGAATATCAAACAGCATTCTCTGATCATCAAGAGTCACAAAGTTAATCGCCACA
CCCTTTCTCCCGAACCTTCCACTTCTTCCGATACGGTGAAGGTAATTCTCTGGCTGAGTT
GGGAGGTCAAAGTTTATGACTAGAGAGACTTGCTGCACATCGATACCACGAGCCAAGAGA
TCGGTTGTGATTAGAACACGAGACGAGCCAGACCTGAACTCTCTCATGATGATGTCTCTG
GTGTTTTGGTCCATGTCCGATGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 28-K105705-0022-171-D05C-8409
GenBank Accession LN498847 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K105705-0022-171-D05C-8409
GGAATGAGCCATTTACATTGAATATATCCATCGGACATGGACAAAACACCAGAGACATCA
TCATGAGAGAGTTCAGGTCTGGCTCGTCTCGTGTTCTAATCACAACCGATCTCTTGGCTC
GTGGTATCGATGTGCAGCAAGTCTCTCTAGTCATAAACTTTGACCTCCCAACTCAGCCAG
AGAATTACCTTCACCGTATCGGAAGAAGTGGAAGGTTCGGGAGAAAGGGTGTGGCGATTA
ACTTTGTGACTCTTGATGATCAGAGAATGCTGTTTGATATTCAGAAATTCTACAATGTGG
TTGTTGAGGAGCTTCCCTCAAACGTGGCTGATTTGCTGTGAAGAAGAAGAAGAAGAAGAA
GAAGAAAGGTTGCTTGTTTCGCAAATTTCTTATTTGATTTTGGACCCA

Last Updated on 06.12.2018 16:11