Sequences from confirmation amplicon


Confirmation sequences for line 171G05 and hit At2g40740 are shown.
Line ID 171G05
Locus At2g40740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16998331
Primers used for PCR IS12 - 8474, show primer details
   
Sequence Name 23-K015962-022-171-G05C-8409
GenBank Accession LN496344 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K015962-022-171-G05C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAATACATCTTTT
CCGATACTACGATTAAAATTTAATCATTAAATGAGTACGTTATTTAGGAAGGACGAAGGA
GAAGAACAAACGGTGTTGGTGGCGGCGCTAAGGACGGGGAACACAGATCTGCCACCCGAC
GATAACCATACTTGGCGTAAATACGGTCAAAAAGAAATTCTTGGCTCTAGGTTTCCTAGG
TAATTTAACTTTAGTTACCAAATAAGTATTTATACTATATCTGAGATTAATATAACCCAA
GTTTAGTTGGAACTTGGAAGACGAAAATAAGCTTTTCGTCCAGAACAAGTCAAAGATTCT
AGATTAGTAATGTAGAATAAGGTCATAATAATATCTTTTCATATATAAGGTCACAAGAAG
TTCCTTTAGTTAATTACATATTTTCCCATCACATCATATGTTGTAATCAGTCTCTTTTGA
TTCTTAACCACCAAGTTTATTAGTGATAGAATGACCAAATAATCCTATTATTAAAGTTCA
GCTGGAAGTTGAAACTTCGATGTTACAAGTACATATCTTTCTCAGATTTCGTTATCAAAT
ACCTTTAATCTAATTCAATCATTAACTACGTACTTTAAAAAACAAAGTTATTAGCTACTA
CTTGATTATCATATAGATGTAAGATTATTAAATTTCTATTTT
   
Sequence Name 24-K015962-022-171-G05C-IS12
GenBank Accession LN496783 [GenBank]
Sequencing primer gene specific primer, IS12: 5'-CCTATAGTACGCCCTGCAAAATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K015962-022-171-G05C-is12
AATTTAATAATCTTACATCTATATGATAATCAAGTAGTAGCTAATAACTTTGTTTTTTAA
AGTACGTAGTTAATGATTGAATTAGATTAAAGGTATTTGATAACGAAATCTGAGAAAGAT
ATGTACTTGTAACATCGAAGTTTCAACTTCCAGCTGAACTTTAATAATAGGATTATTTGG
TCATTCTATCACTAATAAACTTGGTGGTTAAGAATCAAAAGAGACTGATTACAACATATG
ATGTGATGGGAAAATATGTAATTAACTAAAGGAACTTCTTGTGACCTTATATATGAAAAG
ATATTATTATGACCTTATTCTACATTACTAATCTAGAATCTTTGACTTGTTCTGGACGAA
AAGCTTATTTTCGTCTTCCAAGTTCCAACTAAACTTGGGTTATATTAATCTCAGATATAG
TATAAATACTTATTTGGTAACTAAAGTTAAATTACCTAGGAAACCTAGAGCCAAGAATTT
CTTTTTGACCGTATTTACGCCAAGTATGGTTATCGTCGGGTGGCAGATCTGTGTTCCCCG
TCCTTAGCGCCGCCACCAACACCGTTTGTTCTTCTCCTTCGTCCTTCCTAAATAACGTAC
TCATTTAATGATTAAATTTTAATCGTAGTATCGGAAAAGATGTATTCAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGNCAATGAGTATNGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA

Last Updated on 06.12.2018 16:11