Sequences from confirmation amplicon


Confirmation sequences for line 173D01 and hit At2g46270 are shown.
Line ID 173D01
Locus At2g46270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19001156
Primers used for PCR DR16 - 8474, show primer details
   
Sequence Name 55-K018969-022-173-D01C-DR16
GenBank Accession LN508663 [GenBank]
Sequencing primer gene specific primer, DR16: 5'-AGCTTCTTCATCAATCCATTGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K018969-022-173-D01C-DR16
CCTGTACATTTAGTAGGAGTGTCGATGCTCAAAAGCGTCCCCGGCTGCGATAAAGAAGAA
CTATCCATTAAACAGCGAACAAAACCATTTCTCAATCTTTGATGTATCAAACTAGGTATA
CAACTTACAGTTGTTAAAGGTGGATCCTTTTGACCTTGAGGCAGTGATCCCTGTCCACAG
AAACTCAAAGCCAAGTCAGATAAACGATCACGGAGGATTCAACTACGAGATGTTTGAGGA
ACAGAATTACTATATACCATGGGAATACCGGGATGAGCGTAAACTCCTCCTCCATGAGGA
TAAACAGCAGCATAGGGTGCTCCATATGGTGACATCATATGCTGAAAGGGATTTAAGACC
AACAAAACACCAATGAAACGTCTTCCTAATCTTTACCATATTAGCACAAGATGATTCGTG
TTTGTGTTTAGTACCTGAGGATTCCACATGTAAGGAGGAGGAGGATGACCAGATGCAGCC
ATAGCTGAATTGTAATAAGGAGGCATTGCTACTATCCGGTTGGCCTGAGTATATTCAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
   
Sequence Name 56-K018969-022-173-D01C-8409
GenBank Accession LN508970 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K018969-022-173-D01C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAATATACTCAGG
CCAACCGGATAGTAGCAATGCCTCCTTATTACAATTCAGCTATGGCTGCATCTGGTCATC
CTCCTCCTCCTTACATGTGGAATCCTCAGGTACTAAACACAAACACGAATCATCTTGTGC
TAATATGGTAAAGATTAGGAAGACGTTTCATTGGTGTTTTGTTGGTCTTAAATCCCTTTC
AGCATATGATGTCACCATATGGAGCACCCTATGCTGCTGTTTATCCTCATGGAGGAGGAG
TTTACGCTCATCCCGGTATTCCCATGGTATATAGTAATTCTGTTCCTCAAACATCTCGTA
GTTGAATCCTCCGTGATCGTTTATCTGACTTGGCTTTGAGTTTCTGTGGACAGGGATCAC
TGCCTCAAGGTCAAAAGGATCCACCTTTAACAACTGTAAGTTGTATACCTAGTTTGATAC
ATCAAAGATTGAGAAATGGTTTTGTTCGCTGTTTAATGGATAGTTCTTCTTTATCGCAGC
CGGGGACGCTTTTGAGCATCGACACTCCTACTAAATCTACAGGGAACACAGACAATGGAT
T

Last Updated on 06.12.2018 16:11