Sequences from confirmation amplicon


Confirmation sequences for line 174B09 and hit At2g41370 are shown.
Line ID 174B09
Locus At2g41370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17237812
Primers used for PCR AC47 - 8474, show primer details
   
Sequence Name 51-K027911-022-174-B09C-8409
GenBank Accession LN507385 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K027911-022-174-B09C-8409
CATTTATTTAGCCATTGGTAATTATGTAGGTTTTTTTTAGTTGCTATCGCTCATCGTTGT
ACTAATTAATCATTTGATTAACTTTGCCATCGATTAATATCTTATCTTTATACCCTAATT
TCTTCCTAGTACATGCTTTCGTACGTAACTACGTCTATATTTTTTAACTAACATAAGTTA
CTATTACAAATTTACAATGATACATGTTATGTTCGAAAGTTAAGCCTTTCACAATTATAG
TTATTTGACATGATTTTGTTTTATTCGTGATTCGAGAACTGTCTTTTAATTGAAGTTTTT
GTATGTATTTGCATCAGCAATGTCTTATCGGGTGTATAAATATATTCAAATTATCTTTCT
ATACAACACAAATATATCTTTCTTCTTCTCCTCTTTGAAATTTTTGATAGTTTCTATATA
TTTG
   
Sequence Name 52-K027911-022-174-B09C-AC47
GenBank Accession LN507697 [GenBank]
Sequencing primer gene specific primer, AC47: 5'-GATAGAGAATCAGCCACACAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K027911-022-174-B09C-AC47
AATTTCAAGAGGAGAGAAGAAGATTATTTGTGTTGTATAGAAAGATAATTTGAATATATT
TATACACCCGATAAGACATTGCTGATGCAAATACATACAAAAACTTCAATTAAAAGACAG
TTCTCGAATCACGAATAAAACAAAATCATGTCAAATAACTATAATTGTGAAAGGCTTAAC
TTTCGAACATAACATGTATCATTGTAAATTTGTAATAGTAACTTATGTTAGTTAAAAAAT
ATAGACGTAGTTACGTACGAAAGCATGTACTAGGAAGAAATTAGGGTATAAAGATAAGAT
ATTAATCGATGGCAAAGTTAATCAAATGATTAATTAGTACAACGATGAGCGATAGCAACT
AAAAAAAACCTACATAATTACCAATGGCTTAAATAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAAATCTTTATTTCGACGTGTCT

Last Updated on 06.12.2018 16:11