Sequences from confirmation amplicon


Confirmation sequences for line 174C07 and hit At2g25360 are shown.
Line ID 174C07
Locus At2g25360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10805280
Primers used for PCR NR53 - 8474, show primer details
   
Sequence Name 17-K102590-0022-174-C07C-8409
GenBank Accession LN493802 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K102590-0022-174-C07C-8409
GCCATTTACATTGAAATATCCTGGCCAAAATGAAAGTACTCGAATGATGTAGAATAATCG
AGTTAAATAAATATATACCTGCAAGTCATGTGGTTGCAGGCATGAGAAAGTTCGATCATG
TGCCTACACATTTTACACTGACGCCATTTTTTGTCCTTTGCTAGAGACTTTAGCTTCAAC
TCATCAACTAGAGGGTCATGGTGAAGTTTTTTGTACTCAGCACACGACAAATCAGAATGT
GATGGTACTTTGCAATCAATGCAGAAGAGTCCACAACATTTGACACAAGAACGGTCATTG
GATAGGTCGGATTCACTAGAAAGCTCGGTTTTGGACATTAGCATTGAGCAGTTTGGATAT
G
   
Sequence Name 17-K102591-0022-174-C07C-NR53
GenBank Accession LN493803 [GenBank]
Sequencing primer gene specific primer, NR53: 5'-GAGAATCTATTGCCCATATCCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K102591-0022-174-C07C-NR53
GAGCTTTCAGTGAATCCGACCTATCCATGACCGTTCTTGTGTCAAATGTTGTGGACTCTT
CTGCATTGATTGCAAAGTACCATCACATTCTGATTTGTCGTGTGCTGAGTACAAAAAACT
TCACCATGACCCTCTAGTTGATGAGTTGAAGCTAAAGTCTCTAGCAAAGGACAAAAAATG
GCGTCAGTGTAAAATGTGTAGGCACATGATCGAACTTTCTCATGCCTGCAACCACATGAC
TTGCAGGTATATATTTATTTAACTCGATTATTCTACATCATTCGAGTACTTTCATTTTGG
CCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACATTCAC

Last Updated on 06.12.2018 16:11