Sequences from confirmation amplicon


Confirmation sequences for line 174G03 and hit At2g43000 are shown.
Line ID 174G03
Locus At2g43000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17880997
Primers used for PCR MZ18 - 8474, show primer details
   
Sequence Name 21-K017255-022-174-G03C-MZ18
GenBank Accession LN495454 [GenBank]
Sequencing primer gene specific primer, MZ18: 5'-TATTTTCTTTCCACAAAATGGACC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K017255-022-174-G03C-MZ18
ACCAGAGTAGACTTAAAAACGAAAGAATCATGAGAGTAGTATTCTTTGAGATATGTGATA
AAGTTAGTTCACGATAATCCTAATGGAAGTTACTTCACAACCATTTTATGGAATTTACAG
TGGGTCAAATGTGTTCATCTAGTCAAAATTGATATCATTTGGTTTGAATCAACAAAAGTA
TAGCCACTAGAAAATCATCAATTGGTTAATGTATTTTCCAATAAACTAAAAGGCATTCTA
GTAGCTATTGTTGTTCGAATCGGATTCTCTTATAGTTAAATTGAGACTTTTTCTTTTCAT
TGAGTCAATGGCAATCAATGGAAGTTATGTATTTTTCGTGAATTTTCTTTATAAAAATTA
AATAATCATATAGTATAACAAAAAAATGCGTCACGCATTCATAAATTGCTTTCAGATATT
ACTACAGTTGGAATCTATTAAATGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT
   
Sequence Name 22-K017255-022-174-G03C-8409
GenBank Accession LN495899 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K017255-022-174-G03C-8409
AGATTTCCCCGACATGAAGCCATTTACAATTGAATATATCATTTAATAGATTCCAACTGT
AGTAATATCTGAAAGCAATTTATGAATGCGTGACGCATTTTTTTGTTATACTATATGATT
ATTTAATTTTTATAAAGAAAATTCACGAAAAATACATAACTTCCATTGATTGCCATTGAC
TCAATGAAAAGAAAAAGTCTCAATTTAACTATAAGAGAATCCGATTCGAACAACAATAGC
TACTAGAATGCCTTTTAGTTTATTGGAAAATACATTAACCAATTGATGATTTTCTAGTGG
CTATACTTTTGTTGATTCAAACCAAATGATATCAATTTTGACTAGATGAACACATTTGAC
CCACTGTAAATTCCATAAAATGGTTGTGAAGTAACTTCCATTAGGATTATCGTGAACTAA
CTTTATCACATATCTCAAAGAATACTACTCTCATGATTCTTTCGTTTTTAAGTCTACTCT
GGTCTAGTTTTAACAAAAAGTTGGGTCCATTTTGTG

Last Updated on 06.12.2018 16:11