Sequences from confirmation amplicon


Confirmation sequences for line 175D06 and hit At1g74920 are shown.
Line ID 175D06
Locus At1g74920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28139165
Primers used for PCR BK23 - 8474, show primer details
   
Sequence Name 68-K104542-0022-175-D06C-BK23
GenBank Accession LN512010 [GenBank]
Sequencing primer gene specific primer, BK23: 5'-GGTGTCTACTTTGTTAGGCCTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K104542-0022-175-D06C-BK23
ATACGCTCGCAGCCTTGCTTTACCAGCCCATGGGGTGGTGTCAACGCAGTGGGTTTGGAC
GAGAACTTGGAGAATGGTACATTTCTTTTTCTATCTCGAATCTTGTTTTTAAAGACTATA
TCTTGATGGCTTGTACTGAACAAATCTTGGTTATGGCTGGTTTCTTGCGTGCTTCAGGGG
ACTTGATAACTACTTGAGTGTGAAGCAAGTGACTCTCTACACTTCAAACGATCCCTGGGG
ATGGTACAAATCTCCCAACTAAGTAATGAATATGATGGATCAGCAATAAAACAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 84-K104543-0022-175-D06C-8409
GenBank Accession LN514428 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K104543-0022-175-D06C-8409
GCCTTTGTTTATTGCTGATCCATCATATTCATTACTTAGTTGGGAGATTTGTACCATCCC
CAGGGATCGTTTGAAGTGTAGAGAGTCACTTGCTTCACACTCAAGTAGTTATCAAGTCCC
CTGAAGCACGCAAGAAACCAGCCATAACCAAGATTTGTTCAGTACAAGCCATCAAGATAT
AGTCTTTTAAAAACAAGATTCGAGATAGAAAAAGAAATGTACCATTCTCCAAGTTCTCGT
CCAAACCCACTGCGTTTGACACCACCCCATGGGGCTTGGGTAAAGCAAGGCTGCGAGCAG
TTAATCCAGACAATCCCAGCTTCAAAGGCCTAACAAAGTAGACACC

Last Updated on 06.12.2018 16:11