Sequences from confirmation amplicon


Confirmation sequences for line 175F11 and hit At3g27700 are shown.
Line ID 175F11
Locus At3g27700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10259841
Primers used for PCR BU17 - 8474, show primer details
   
Sequence Name 01-K104372-0022-175-F11C-BU17
GenBank Accession LN486560 [GenBank]
Sequencing primer gene specific primer, BU17: 5'-CATTTTCCATTTGTGCAGAGTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K104372-0022-175-F11C-BU17
CCTCATCTGGCAGCTTCTTCTAAACAAGTGCCCGCTCAGTTTGGGGGTGCCAGTTTCATG
AATCCTAAAGGAGCTCATGGGAGGACTAACGAGGGGGGAATGGCTGTTGATGGTTTGGGT
TATGGGGATGCATATCCTAGTGCTGGTGGAACTGACTTTTACGATCCTGATCAACCTTTG
TGGAATAATAGTACTGGTGAAACTTCAGGTGCAATATCTACACTCAATTCTCATGGGGTT
GATGAGAATGTGGCTCCACTGGATGACAGTAACCAAGACGCCGCTGAAAATGGGTGTGGT
ATTAGAGACTCTAGAAGCACAATCTACATGGCTTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAATTTT
   
Sequence Name 17-K104373-0022-175-F11C-8409
GenBank Accession LN493836 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K104373-0022-175-F11C-8409
ATTGAAGCCTGTAGATTGTGCTTCTAGAGTCTCTAATACCACACCCATTTTCAGCGGCGT
CTTGGTTACTGTCATCCAGTGGAGCCACATTCTCATCAACCCCATGAGAATTGAGTGTAG
ATATTGCACCTGAAGTTTCACCAGTACTATTATTCCACAAAGGTTGATCAGGATCGTAAA
AGTCAGTTCCACCAGCACTAGGATATGCATCCCCATAACCCAAACCATCAACAGCCATTC
CCCCCTCGTTAGTCCTCCCATGAGCTCCTTTAGGATTCATGAAACTGGCACCCCCAAACT
GAGCGGGCACTTGTTTAGAAGAAGCTGCCAGATGAGGTGCACCAGGGACTGAAACGGGAA
GGTTAAACTGTGATAGACTCTGCACAAAGGGAAAATG

Last Updated on 06.12.2018 16:11