Sequences from confirmation amplicon


Confirmation sequences for line 175G10 and hit At2g20230 are shown.
Line ID 175G10
Locus At2g20230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8727352
Primers used for PCR EO04 - 8474, show primer details
   
Sequence Name 32-K018601-022-175-G10C-EO04
GenBank Accession LN500385 [GenBank]
Sequencing primer gene specific primer, EO04: 5'-ATGCTCGAATCTTGTTTCTTGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K018601-022-175-G10C-EO04
GGAACAGTTTAACATCTGTGTTCTGTAGGATCTTCCGTATGATCCAACTGGAGAACTTAG
TAGTCTTAGGGCTTTCATTGAAGAAAACATCGATATATGCAAATGGGTGGGGATTGCTGT
GGTAGCAGTCCAGGTAATTCAATTCAGTCTTTATAAAATTTGAGAATTGTCATGATATAT
ATATTCTCTCTAGAGAGTTTAAAGAGAAGACATGAATATTTCTTGTGTCTTTCAGCTACT
GTCATTGCTACTAGCCATGGTTCTCAGAGCGATGGTTTCAACCCCGAAACCCGAACTTGA
CGAAGAGGAAGATGATGAGAACCCAAGGAGTAGAACTTGGGACCCACTTCTTGGTCCGCA
AGGAAACCAAGCGCCTGCTGGCTCAAGCAAGATTGAAAACTGGAGCTCCCGAATCAGAGA
AAAGGTAATGCTGATCATTTTTTCAGTTTCTTGATACTTTACGGATTGGATAATGCTTTG
TGAATCTGCTGATGATAAAAACTGTTTCGTTTTGAAAATTTCCTTTTGCAGTATGGTATG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
   
Sequence Name 33-K018601-022-175-G10C-8409
GenBank Accession LN500803 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K018601-022-175-G10C-8409
AAGCCATTTACAATTGAATATATCATACCATACTGCAAAAGGAAATTTTCAAAACGAAAC
AGTTTTTATCATCAGCAGATTCACAAAGCATTATCCAATCCGTAAAGTATCAAGAAACTG
AAAAAATGATCAGCATTACCTTTTCTCTGATTCGGGAGCTCCAGTTTTCAATCTTGCTTG
AGCCAGCAGGCGCTTGGTTTCCTTGCGGACCAAGAAGTGGGTCCCAAGTTCTACTCCTTG
GGTTCTCATCATCTTCCTCTTCGTCAAGTTCGGGTTTCGGGGTTGAAACCATCGCTCTGA
GAACCATGGCTAGTAGCAATGACAGTAGCTGAAAGACACAAGAAATATTCATGTCTTCTC
TTTAAACTCTCTAGAGAGAATATATATATCATGACAATTCTCAAATTTTATAAAGACTGA
ATTGAATTACCTGGACTGCTACCACAGCAATCCCCACCCATTTGCATATATCGATGTTTT
CTTCAATGAAAGCCCTAAGACTACTAAGTTCTCCAGTTGGATCATACGGAAGATCCTACA
GAACACAGATGTTAAACTGTTACAAACAAGTCAAGGAAACAAGAAACAAGAAACAAGATT
CGAGCA

Last Updated on 06.12.2018 16:11