Sequences from confirmation amplicon


Confirmation sequences for line 176H03 and hit At3g55120 are shown.
Line ID 176H03
Locus At3g55120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20430445
Primers used for PCR MR28 - 8474, show primer details
   
Sequence Name 07-K015287-022-176-H03C-8409
GenBank Accession LN489033 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K015287-022-176-H03C-8409
CATACGCAACCTACAAAACAGTATAGTTCACAACAAACACATGTCAACAAAGTATTCAAA
CCTACACGTTACAACTACTATCGTTCCAAACTTCCAAACGCCAACTTTTACAATAGTACT
TACATTTAAAAAACCTAAAACAGTACCTTCGCAAGTGGTTTAAATGCGAAAGCATGTCAA
GTATCTCATTCCAATTTGATATTTGCACTAACGATTAACAAAATTCAAGCAAAGTATAAA
TCAAGCAAAAATTACCGTAAGAGAGCCGGTAGGGGAGAGAGCGAAGAGGATCGATGAACC
GGGAGGGAATGTTTCTTCCTTGAAGATCTCCAAGAACTTCTCCACAGCTTTAGCTTCACA
GTCCGTATAAAGCCCTAATTGTTTCCATATAAGCC
   
Sequence Name 08-K015287-022-176-H03C-MR28
GenBank Accession LN489498 [GenBank]
Sequencing primer gene specific primer, MR28: 5'-GTGGCTATATGGAAACAATTAGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K015287-022-176-H03C-MR28
TTTATACGGACTGTGAAGCTAAAGCTGTGGAGAAGTTCTTGGAGATCTTCAAGGAAGAAA
CATTCCCTCCCGGTTCATCGATCCTCTTCGCTCTCTCCCCTACCGGCTCTCTTACGGTAA
TTTTTGCTTGATTTATACTTTGCTTGAATTTTGTTAATCGTTAGTGCAAATATCAAATTG
GAATGAGATACTTGACATGCTTTCGCATTTAAACCACTTGCGAAGGTACTGTTTTAGGTT
TTTTAAATGTAAGTACTATTGTAAAAGTTGGCGTTTGGAAGTTTGGAACGATAGTAGTTG
TAACGTGTAGGTTTGAATACTTTGTTGACATGTGTTTGTTGTGAACTATACTGTTTTGTA
GGTTGCGTATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTA
CATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAA
TTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGGGGGCTT
AGATGAGAAACTTCACGATCGATGCCTTGA
Primers used for PCR 36vq - 3144, show primer details
   
Sequence Name 64-K117894-0022-176-H03C-36vq
GenBank Accession LN511289 [GenBank]
Sequencing primer gene specific primer, 36vq: 5'-ACCTGTCTGACTCATCACAACAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K117894-0022-176-H03C-36vq
CTCGTTGCTTCTTTGTTTTGAGCTCGAAACGTTGTTGCTTATGATCTGTTTCACTCTTCT
CTATTCTCTCTTCTTCAACAGAATTAGACTCCCCAAAAATGTAACCGAAAAGTCCAAAAC
CAATGCTAAACGCAACCCACAAAAAAGCGTTAGATTCTCTGCTTTAATACAACCGAGTAT
TCCAATTTATTGGACCATAATTTCCTTATGTCAAGCACCATCCTTTAATAACGTTTTTGG
CGAATAAGGTTTATCAGAGTTTTTTGGAATCTGATTCCGAGTAACTCCCATCTAAACCTC
AACGGTTGAATCATCTTTGACATATATATCATCAAATCAAGTTAGAATAGCATTTCAAAG
AAAAACACCGAAATTCCTAAACTTTATTATAATGTAAATGCATGTTCTTTATTCTCCACT
TGAGTACCGCATAAGAAATTAGATATAGAAAACATAAGAAATAGAAAATGACAAAAACAC
AAGAAAAATCTATCATTCTCAGTTCTCTTTGGCTAGTTTTTCCTCAACAGAGTGATCACT
AACTTCCTTTTCGTCCTTGTTCTTCATCATTAGCTGAGATAATCTTTCTGCAACACTTAA
CCTAGTGCCAGGTGACACACCGTTCTTCCCGATGATAGATTCCAGAACCGCCTCCGCCAA
CAATTTGTTCTCGATCACAGCGATCCCGGTTTCAGGAAACGACACTGATCCCCGGGTACC
CTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGT
GGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATC
   
Sequence Name 53-K117893-0022-176-H03C-35St
GenBank Accession LN508286 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K117893-0022-176-H03C-35St
CCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGTGTCGTTTCCTGAAACCGGGATCGCTGTGATCGAGAACAAATTGTTGGCGG
AGGCGGTTCTGGAATCTATCATCGGGAAGAACGGTGTGTCACCTGGCACTAGGTTAAGTG
TTGCAGAAAGATTATCTCAGCTAATGATGAAGAACAAGGACGAAAAGGAAGTTAGTGATC
ACTCTGTTGAGGAAAAACTAGCCAAAGAGAACTGAGAATGATAGATTTTTCTTGTGTTTT
TGTCATTTTCTATTTCTTATGTTTTCTATATCTAATTTCTTATGCGGTACTCAAGTGGAG
AATAAAGAACATGCATTTACATTATAATAAAGTTTAGGAATTTCGGTGTTTTTCTTTGAA
ATGCTATTCTAACTTGATTTGATGATATATATGTCAAAGATGATTCAACCGTTGAGGTTT
AGATGGGAGTTACTCGGAATCAGATTCCAAAAAACTCTGATAAACCTTATTCGCCAAAAA
CGTTATTAAAGGATGGTGCTTGACATAAGGAAATTATGGTCCAATAAATTGGAATACTCG
GTTGTATTAAAGCAGAGAATCTAACGCTTTTTTGTGGGTTGCGTTTAGCATTGGTTTTGG
ACTTTTCGGTTACATTTTTGGGGAGTCTAATTCTGTTGAAGAAGAGAGAATAGAGA

Last Updated on 06.12.2018 16:11