Sequences from confirmation amplicon


Confirmation sequences for line 178A08 and hit At2g27930 are shown.
Line ID 178A08
Locus At2g27930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11893040
Primers used for PCR 03vz - 8474, show primer details
   
Sequence Name 26-K118126-0022-178-A08C-03vz
GenBank Accession LN498124 [GenBank]
Sequencing primer gene specific primer, 03vz: 5'-TCCTTATTCTTCCTCATTCCTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K118126-0022-178-A08C-03vz
ATTTCCGTTATGCAAATGTACCATATACTTTTTTTTTGAGCAAAATGTACCATATACATA
TATACAAACATCTAACCTCGTATATATTTTCTTAAGATACATTATATGAGGCAAACAACA
TGTATTAAGCGAGTTTGAATATAGTTGATTCAATGAATTTACCATACAAGTAACTAAACA
TTATACGGTGTATCCTTAAACCGATTCCTCATTTTATATCGGACAAATAAATGCGAAGAT
ATTAGTAATAATGATGAAAATATGATACATTTCATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAAGGTTATA
   
Sequence Name 10-K118125-0022-178-A08C-8409
GenBank Accession LN490859 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K118125-0022-178-A08C-8409
CCATTTACATTGAATATGAAATGTATCATATTTTCATCATTATTACTAATATCTTCGCAT
TTATTTGTCCGATATAAAATGAGGAATCGGTTTAAGGATACACCGTATAATGTTTAGTTA
CTTGTATGGTAAATTCATTGAATCAACTATATTCAAACTCGCTTAATACATGTTGTTTGC
CTCATATAATGTATCTTAAGAAAATATATACGAGGTTAGATGTTTGTATATATGTATATG
GTACATTTTGCTCAAAAAAAAAGTATATGGTACATTTGCATAACGGAAATGTATGATGTG
ATCATAATAAAAATGTCAGGTTGAAGGAATGAGGAAGAATAAGGA

Last Updated on 06.12.2018 16:11