Sequences from confirmation amplicon


Confirmation sequences for line 179B10 and hit At5g15630 are shown.
Line ID 179B10
Locus At5g15630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5086201
Primers used for PCR EP20 - 8474, show primer details
   
Sequence Name 16-K102173-0022-179-B10C-8409
GenBank Accession LN493377 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K102173-0022-179-B10C-8409
TGAACTCATTTACGGATCCACAACTATTCAACTTCAACCAACCATTGAATGACTAATAAC
ATTCTTAAATCTTTTTGATACAGATGATACTGGAATGTTCTATGGAACGAAGTTTTACAA
TGATTTATTAATGGAAGCTGGACCTTCAGGGAATGTGCAATCAGAGGTTTTGCTACAGAA
AGATCAAAAGACTTTTACTTTCAAGCAAGGTTGGGCTTTTCCTAGAAAAGTTTACTTTAA
TGGTGATGAATGTATGTTACCTCCACCAGATTCATACCCTTTTCTACCAAACTCTGCACA
AGGGAACTTTGCTTCGTTCTCACTCACCATTCTTCTTCTCCTATTCATCTCAATATGGTG
ATTTGATCTGATCCTTCTGGGTTTTAAAACACTGAACCAGAACTGTTTTTTGTAAACTCA
TTGCAAAGATTGTAAAAGAAAAAAAGGAGTTTTGTGAGAGGAAATGATTAGAGATGTCAA
AGGCTTT
   
Sequence Name 16-K102177-0022-179-B10C-EP20
GenBank Accession LN493378 [GenBank]
Sequencing primer gene specific primer, EP20: 5'-AAAGCCTTGCACATCTCTAATCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K102177-0022-179-B10C-EP20
CTCTTTTTTCTTTACATCTTTGCAATGAGTTTACAAAAAACAGTTCTGGTTCAGTGTTTT
AAAACCCAGAAGGATCAGATCAAATCACCATATTGAGATGAATAGGAGAAGAAGAATGGT
GAGTGAGAACGAAGCAAAGTTCCCTTGTGCAGAGTTTGGTAGAAAAGGGTATGAATCTGG
TGGAGGTAACATACATTCATCACCATTAAAGTAAACTTTTCTAGGAAAAGCCCAACCTTG
CTTGAAAGTAAAAGTCTTTTGATCTTTCTGTAGCAAAACCTCTGATTGCACATTCCCTGA
AGGTCCAGCTTCCATTAATAAATCATTGTAAAACTTCGTTCCATAGAACATTCCAGTATC
ATCTGTATCAAAAAGATTTAAGAATGTTATTAGTCATTCAATGGTTGGTTTGAAGTTGAA
TAGTTGTGGATCCGTAAATGAGTATCAGGATCAGCAATGGCTTCATGATTTCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATATCCGCAGCGTT

Last Updated on 06.12.2018 16:11