Sequences from confirmation amplicon


Confirmation sequences for line 179E12 and hit At3g28910 are shown.
Line ID 179E12
Locus At3g28910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10912028
Primers used for PCR RS07 - 8474, show primer details
   
Sequence Name 16-K016598-022-179-E12C-8409
GenBank Accession LN493209 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K016598-022-179-E12C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATAGGTAAAAAATAATAATG
ACTTTTCAATTTCTCTAATTTTGTCTCTTCACACATTTTCTTTCGAAAATTAGAAGATTT
GTTTTGATTTTTAATAGAGAGCATTTAATATTTGGTGTAGATGGGCTGCAATTGCGTCAT
ATCTTCCACAAAGGACAGACAATGACATTAAGAACTATTGGAACACTCATTTGAAGAAGA
AACTCAACAAAGTCAATCAAGATTCTCATCAAGAACTTGACCGTTCCTCGCTCTCATCTT
CACCATCGTCTTCTTCTGCTAATTCCAACTCAAACATCTCAAGAGGCCAATGGGAAAGGC
GACTTCAAACCGATATCCACTTGGCGAAAAAGGCTCTCTCTGAGGCTTTATCTCCTGCCG
TTGCACCAATCATTACATCTACAGTGACAACAACGTCTTCCTCTGCTGAATCAAGACGCT
CTACTTCCTCAGCTAGCGGGTTTCTTAGGACGCAAGAAACATCTACAACTTATGCCTCAA
GCACCGAAAATATCGCGAAATTGCTCAAAGGGTGGGTGAAAAACTCGCCGAAGACTCAAA
ACTCCGCGGATCAAATCGCTTCTACAGAGGTAAAAGAAGTGATCAAGAGTGATGATGGGA
AGGAGTGTGCAGGGGCATTTCAGTCATTTTCTGAGTTTGATCACTCATATCAACAGGCTG
GTGTTTCACCTGATCATGAGACCAAACCAGACATAACTGGATGCTGCAG
   
Sequence Name 20-K016598-022-179-E12C-RS07
GenBank Accession LN494997 [GenBank]
Sequencing primer gene specific primer, RS07: 5'-CACTTCTCAAACAAAGACCATTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K016598-022-179-E12C-rs07
GTTACTGCAGCATCCAGTTATGTCTGGTTTGGTCTCATGATCAGGTGAAACACCAGCCTG
TTGATATGAGTGATCAAACTCAGAAAATGACTGAAATGCCCCTGCACACTCCTTCCCATC
ATCACTCTTGATCACTTCTTTTACCTCTGTAGAAGCGATTTGATCCGCGGAGTTTTGAGT
CTTCGGCGAGTTTTTCACCCACCCTTTGAGCAATTTCGCGATATTTTCGGTGCTTGAGGC
ATAAGTTGTAGATGTTTCTTGCGTCCTAAGAAACCCGCTAGCTGAGGAAGTAGAGCGTCT
TGATTCAGCAGAGGAAGACGTTGTTGTCACTGTAGATGTAATGATTGGTGCAACGGCAGG
AGATAAAGCCTCAGAGAGAGCCTTTTTCGCCAAGTGGATATCGGTTTGAAGTCGCCTTTC
CCATTGGCCTCTTGAGATGTTTGAGTTGGAATTAGCAGAAGAAGACGATGGTGAAGATGA
GAGCGAGGAACGGTCAAGTTCTTGATGAGAATCTTGATTGACTTTGTTGAGTTTCTTCTT
CAAATGAGTGTTCCAATAGTTCTTAATGTCATTGTCTGTCCTTTGTGGAAGATATGACGC
AATTGCAGCCCATCTACACCAAATATTAAATGCTCTCTATTAAAAATCAAAACAAATCTT
CTAATTTTCGAAAGAAAATGTGTGAAGAGACAAAATTAGAGAAATTGAAAAGTCATTATT
ATTTTTTACCTATATTC

Last Updated on 06.12.2018 16:11