Sequences from confirmation amplicon


Confirmation sequences for line 179H01 and hit At2g19190 are shown.
Line ID 179H01
Locus At2g19190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8326768
Primers used for PCR IS01 - 8474, show primer details
   
Sequence Name 05-K015958-022-179-H01C-8409
GenBank Accession LN488137 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K015958-022-179-H01C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGATTGATTTTCACAGAGAAAACACACACTT
CATTGATTGATTTTCGCATGTTAGATTTAGTTAAATGTAATTTCAAGATGGGACTTCCCG
AACCTTGCGCTGCATCTAATGATATCTTCAACCTCTCTTCCCAGCTCAAGATAAATGACC
TTTTACCTTCACAAAATGAAACAAAACCAAAGTTCCTTTCTCAGTACATTATGAACATAA
GATTAGTGAACAAAAGAAGAATCATTAATTAACCTGCCAAATAGTCTCCTAAGTTCTCAT
TAGCCATATACTCATAGATAAGCACCATGTGGTTTATTTCGTTGCAATATCCAACAAGAG
AAGTCAGGTTCGTGTGATGAACTCTCATGAGAAGGTCAACCTGCGATTCTCAACTAGATT
AGTTAGGTGAAAAAGTTTGAGATAAATTAAGATTAAGTCCTTGAAAGGTACGCAAAAAAA
ACCTCTGCTCGAAACTCTTTGTAGCCTTGAGCTGATTCTTCAGAGAGTACCTTGACAGCA
ACTTGTTC
   
Sequence Name 06-K015958-022-179-H01C-IS01
GenBank Accession LN488579 [GenBank]
Sequencing primer gene specific primer, IS01: 5'-TCATAAATGGAGAACAAGTTGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K015958-022-179-H01C-is01
GTACTCTCTGAAGAATCAGCTCAAGGCTACAAAGAGTTTCGAGCAGAGGTTTTTTTTGCG
TACCTTTCAAGGACTTAATCTTAATTTATCTCAAACTTTTTCACCTAACTAATCTAGTTG
AGAATCGCAGGTTGACCTTCTCATGAGAGTTCATCACACGAACCTGACTTCTCTTGTTGG
ATATTGCAACGAAATAAACCACATGGTGCTTATCTATGAGTATATGGCTAATGAGAACTT
AGGAGACTATTTGGCAGGTTAATTAATGATTCTTCTTTTGTTCACTAATCTTATGTTCAT
AATGTACTGAGAAAGGAACTTTGGTTTTGTTTCATTTTGTGAAGGTAAAAGGTCATTTAT
CTTGAGCTGGGAAGAGAGGTTGAAGATATCATTAGATGCAGCGCAAGGTTCGGGAAGTCC
CATCTTGAAATTACATTTAACTAAATCTAACATGCGAAAATCAATCAATGAAGTGTGTGT
TTTCTCTGTGAAAATCAATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATTGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATTTNGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTAC
Primers used for PCR IS01 - 8474, show primer details
   
Sequence Name 57-K110039-0022-179-H01C-IS01
GenBank Accession LN509447 [GenBank]
Sequencing primer gene specific primer, IS01: 5'-TCATAAATGGAGAACAAGTTGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K110039-0022-179-H01C-IS01
CTCAGGCTAAAAGAGTTTCGAGCAGAGGTTTTTTTTGCGTACCTTTCAAGGACTTAATCT
TAATTTATCTCAAACTTTTTCACCTAACTAATCTAGTTGAGAATCGCAGGTTGACCTTCT
CATGAGAGTTCATCACACGAACCTGACTTCTCTTGTTGGATATTGCAACGAAATAAACCA
CATGGTGCTTATCTATGAGTATATGGCTAATGAGAACTTAGGAGACTATTTGGCAGGTTA
ATTAATGATTCTTCTTTTGTTCACTAATCTTATGTTCATAATGTACTGAGAAAGGAACTT
TGGTTTTGTTTCATTTTGTGAAGGTAAAAGGTCATTTATCTTGAGCTGGGAAGAGAGGTT
GAAGATATCATTAGATGCAGCGCAAGGTTCGGGAAGTCCCATCTTGAAATTACATTTAAC
TAAATCTAACATGCGAAAATCAATCAATGAAGTGTGTGTTTTCTCTGTGAAAATCAATCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CA
   
Sequence Name 41-K110038-0022-179-H01C-8409
GenBank Accession LN504111 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K110038-0022-179-H01C-8409
GAGCATTTACATTGATTGATTTTCCAGAGAAAACACACACTTCATTGATTGATTTTCGCA
TGTTAGATTTAGTTAAATGTAATTTCAAGATGGGACTTCCCGAACCTTGCGCTGCATCTA
ATGATATCTTCAACCTCTCTTCCCAGCTCAAGATAAATGACCTTTTACCTTCACAAAATG
AAACAAAACCAAAGTTCCTTTCTCAGTACATTATGAACATAAGATTAGTGAACAAAAGAA
GAATCATTAATTAACCTGCCAAATAGTCTCCTAAGTTCTCATTAGCCATATACTCATAGA
TAAGCACCATGTGGTTTATTTCGTTGCAATATCCAACAAGAGAAGTCAGGTTCGTGTGAT
GAACTCTCATGAGAAGGTCAACCTGCGATTCTCAACTAGATTAGTTAGGTGAAAAAGTTT
GAGATAAATTAAGATTAAGTCCTTGAAAGGTACGCAAAAAAAACCTCTGCTCGAAACTCT
TTGTAGCCTTGAGCTGATTCTTCAGAGAGTACCTTGACAGCAACTTGTTCCCAATTTATG
A

Last Updated on 06.12.2018 16:11