Sequences from confirmation amplicon


Confirmation sequences for line 180D12 and hit At2g37340 are shown.
Line ID 180D12
Locus At2g37340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15670891
Primers used for PCR CK03 - 8474, show primer details
   
Sequence Name 41-K017716-022-180-D12C-CK03
GenBank Accession LN503901 [GenBank]
Sequencing primer gene specific primer, CK03: 5'-GCCATCAAAGTTAAGGAGACTCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K017716-022-180-D12C-CK03
CTCTAGGGGAAGTGCGGTTTGGCTCGTAATCATCATCAACAGGGCTCCGGTCCTCTTTCG
AACTGTCACCACCGGCTCCTACAACAGGACTGACAACAGAGTTTCTGCCATTGTCTTGGG
GGCTACCACCGTCACGGTCAGATCCGACTTCCTTTTGAAGCTTTGGTGATGGTGGTGGGC
TCCCGTCTATGATCTTTGGGCTGCCTTCATCATCAAGAACCGGACTACGATCTCTGGCTC
TTGGCGATAGAGAGTCATCCATCCGCTTTGGACTGCGTGATCTCTCCTCCACACTCCTCT
CTCTTCTTCTCACCGGGGATCGTGATCGGCTGACCATAAGGAAAATAAGAAGTCAATCAG
AGAAAGCAGTTACTCGGATTCATGCTTTCAGGTTCATACATAAATCTAATGCATGTAAAC
ACCTAAACATAAACCAAAATTTTGAAGTTTACACGGGCAAATACTAACCTGTAGCTTCGG
CTACGGCTAAGACTCCGGCTTGGTGTCCTGGAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTAC
   
Sequence Name 42-K017716-022-180-D12C-8409
GenBank Accession LN504292 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K017716-022-180-D12C-8409
ATCCATGTAGATTTCCCGGACATGAAGCCATTTCCAGGACACCAAGCCGGAGTCTTAGCC
GTAGCCGAAGCTACAGGTTAGTATTTGCCCGTGTAAACTTCAAAATTTTGGTTTATGTTT
AGGTGTTTACATGCATTAGATTTATGTATGAACCTGAAAGCATGAATCCGAGTAACTGCT
TTCTCTGATTGACTTCTTATTTTCCTTATGGTCAGCCGATCACGATCCCCGGTGAGAAGA
AGAGAGAGGAGTGTGGAGGAGAGATCACGCAGTCCAAAGCGGATGGATGACTCTCTATCG
CCAAGAGCCAGAGATCGTAGTCCGGTTCTTGATGATGAAGGCAGCCCAAAGATCATAGAC
GGGAGCCCACCACCATCACCAAAGCTTCAAAAGGAAGTCGGATCTGACCGTGACGGTGGT
AGCCCCCAAGACAATGGCAGAAACTCTGTTGTCAGTCCTGTTGTAGGAGCCGGTGGTGAC
AGTTCGAAAGAGGACCGGAGCCCTGTTGATGATGATTACGAGCCAAACCGCACTTCCCCT
AGAGGAAGTGAGTCTCCT

Last Updated on 06.12.2018 16:11