Sequences from confirmation amplicon


Confirmation sequences for line 181C04 and hit At5g46540 are shown.
Line ID 181C04
Locus At5g46540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18878640
Primers used for PCR PA37 - 8474, show primer details
   
Sequence Name 36-K037018-022-181-C04C-PA37
GenBank Accession LN502134 [GenBank]
Sequencing primer gene specific primer, PA37: 5'-GAATATCGAAGCAGCAGAGTCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K037018-022-181-C04C-PA37
TAGTTTGAGTCCTCCTACGGCTGTCAAGTTAACGCGAAGAACCCTGAAATGTTAAAAAAA
ACACTCAAACATCACAAACCCGAACCAAACCGGATCATAATCGAACCTGATTCTACTCTT
TTATACCTGAAAGAATTCTCCGAACGTTGCCCTCCTGTTCTGAATCAACCAGGAACCGCC
GAGAAAGCATACGGACTCGATGACATAGAGAGCCAAGTATGAGCCACCATAGCACAAACC
ACTCACCAGTCCTAATTTGAAGCCTTGTTGCTTTGGTTCGTCACATTTCTCTTGGTATAA
ATCCATCACTTTATCCTCTGCGCAGAACGATGCAACTGTTCTTATGCTGCTCACCGCGTC
ACTCGCAACCTGGCTAGCCTCTTCATATTTTCCCTAAATCGAAGTAAGAAATCATGTTAG
ACTATATAGAACCAGAGTTTAACCAAGGTTGTTGAATACTAACTACTAACTCTTGCTTTT
GCTCCAAAACCCGGATCTACATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTA
TTTCGACGTGTCTACA
   
Sequence Name 35-K037018-022-181-C04C-8409
GenBank Accession LN501741 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K037018-022-181-C04C-8409
GCCATGTAGATCCGGGTTTTGGAGCAAAGCAAGAGTTAGTAGTTACCTTTAACAACCTTG
GTTAAACTCTGGTTCTATATAGTCTCATATGATTTCTTACTTCGATTTAGGGAAAATATG
AAGAGGCTAGCCAGGTTGCGAGTGACGCGGTGAGCAGCATAAGAACAGTTGCATCGTTCT
GCGCAGAGGATAAAGTGATGGATTTATACCAAGAGAAATGTGACGAACCAAAGCAACAAG
GCTTCAAATTAGGACTGGTGAGTGGTTTGTGCTATGGTGGCTCATACTTGGCTCTCTATG
TCATCGAGTCCGTATGCTTTCTCGGCGGTTCCTGGTTGATTCAGAACAGGAGGGCAACGT
TCGGAGAATTCTTTCAGGTATAAAAGAGTAGAATCAGGTTCGATTATGATCCGGTTTGGT
TCGGGTTTGTGATGTTTGAGTGTTTTTTTTAACATTTCAGGTTTTCTTCGCGTTAACTTT
GACAGCCGTAGGAGTGACTCAAACTAGTACTATGGCACCTGACATAAACAAAGCAAAAGA
CTCTGCTGCTTCCGATATTCA

Last Updated on 06.12.2018 16:11