Sequences from confirmation amplicon


Confirmation sequences for line 181D04 and hit At1g73230 are shown.
Line ID 181D04
Locus At1g73230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27541313
Primers used for PCR PE45 - 8474, show primer details
   
Sequence Name 46-K022405-022-181-D04C-8409
GenBank Accession LN505732 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K022405-022-181-D04C-8409
CATCATATTCATCCTGTTTTTCAAAACAAACACACAAAGCTAAACCCATTTAGCCCAAAA
TGTATCTTATAAATTAAGCATATACAATAACTAATACTGTTATTAATTAGCCAATGACAA
AAACAGTAATACTGAGCAATAGTCACAACCCACAAATTGATTACACTAGGATTATGAGAA
CAGTGCAGCGAGAAGAACAAAGCTCGTATTGTTACAATGAGCTTAACCTAAAGTTTCAAT
TTTTTCAATCGATTAGGTCACCATTACACAAACATTACTTTTAACTAACTAACAAGTTAT
TGGAAAACAAGATAACCAATATCAAAAACAGTAATCAATGAAAAAAAACATATGAATCGA
GGAAGAACAAAAACAAAAAAATGATCTTGACCCTTGTGCTAAATCAGAAGGAAACAATCG
AATATCTTGGAAATGAAATGAGTGAACGATTTGCTGAAGTGGGCTTACCTTGTTAATCGA
TTTCTAGGGTTCTTCGTTGGTCGAAGGGTATAAGAAGAAGATAAGGGAAGAGAGAACAGA
GG
   
Sequence Name 47-K022405-022-181-D04C-PE45
GenBank Accession LN506060 [GenBank]
Sequencing primer gene specific primer, PE45: 5'-TGGATCTCTCTCTCCTCTGTTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K022405-022-181-D04C-PE45
CCTTATCTTCTTCTTATACCCTTCGACCAACGAAGAACCCTAGAAATCGATTAACAAGGT
AAGCCCACTTCAGCAAATCGTTCACTCATTTCATTTCCAAGATATTCGATTGTTTCCTTC
TGATTTAGCACAAGGGTCAAGATCATTTTTTTGTTTTTGTTCTTCCTCGATTCATATGTT
TTTTTTCATTGATTACTGTTTTTGATATTGGTTATCTTGTTTTCCAATAACTTGTTAGTT
AGTTAAAAGTAATGTTTGTGTAATGGTGACCTAATCCATTGAAAAAATTGAAACTTTAGG
TTAAGCTCATTGTAACAATACCAGCTTTGTTCTTCTCGCTGCACTGGTCTCATAATCCTA
ATGGAATCCATTTGGGGGTTGGGACCATTGGTCAATAATACCGGTTTTGGCCTTGGCTAA
AT
Primers used for PCR FM89 - 8474, show primer details
   
Sequence Name 39-K032007-022-181-D04C-8409
GenBank Accession LN503252 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K032007-022-181-D04C-8409
CGGAAGGGGACAGTAAGAAGGATACTCATTTATGTCTTTTCTGTGAATTTGTGTTTTTGC
CATTTTTCATGTTATATTCTCTAAACATTTTGACTCTCATTTCAGAAAGAAGAAGGCTGT
TCACAAGACCACTACAACCGATGACAAGAGGCTCCAGAGCACTCTTAAGAGAGTTGGAGT
CAATTCCATTCCCGCCATTGAAGAAGTTAACATTTTTAAGGATGATGTAGTCATTCAGTT
CATTAACCCTAAAGGTAAAACATCAAATTTTATGGCTTTATAAATCTCAGGTTTCGTATA
AAATTTATTGTATTAATGGTTGTTGTGTCTCTGTTTTAATGTTGCTATCACAGTTCAAGC
TTCAATTGCTGCTAACACATGGGTTGTGAGTGGTACACCACAGACGAAAAGTAAGTGTTT
GTATCCTCATTGTTGATCCAATACTATTCTCGGTTTATGCATAGTCTAATCTCGGAATGT
GATTTATCCT
   
Sequence Name 40-K032007-022-181-D04C-FM89
GenBank Accession LN503605 [GenBank]
Sequencing primer gene specific primer, FM89: 5'-CAGGATAATTCACATTCCGAGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K032007-022-181-D04C-FM89
GGATCACATGAGGATACAAACACTTACTTTTCGTCTGTGGTGTACCACTCACAACCCATG
TGTTAGCAGCAATTGAAGCTTGAACTGTGATAGCAACATTAAAACAGAGACACAACAACC
ATTAATACAATAAATTTTATACGAAACCTGAGATTTATAAAGCCATAAAATTTGATGTTT
TACCTTTAGGGTTAATGAACTGAATGACTACATCATCCTTAAAAATGTTAACTTCTTCAA
TGGCGGGAATGGAATTGACTCCAACTCTCTTAAGAGTGCTCTGGAGCCTCTTGTCATCGG
TTGTAGTGGTCTTGTGAACAGCCTTCTTCTTTCTGAAATGAGAGTCAAAATGTTTAGAGA
ATATAACATGAAAAATGGCAAAAACACAAATTCACAGAAAAGACATAAATGAGTATACCT
TCTTACTGTCCCCTTTCCGCCAGTGCGGACAGTGTTAGCCATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11