Sequences from confirmation amplicon


Confirmation sequences for line 181H05 and hit At1g60920 are shown.
Line ID 181H05
Locus At1g60920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22430460
Primers used for PCR MK04 - 8474, show primer details
   
Sequence Name 41-K119268-0022-181-H05C-MK04
GenBank Accession LN504273 [GenBank]
Sequencing primer gene specific primer, MK04: 5'-CTGATCTGTTATCTTGGTTTGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K119268-0022-181-H05C-MK04
CGTGATCGACAGAGGAATGACCGAAAGAGTAGAAAGAAGCATGGGAGTGGGAAGAAAGAG
GAGTTGCTAAGATTGCGATTTGAGTCCCCGGAGAGAGGAGACAGAGCTCTGAAGCTTTGT
GGAAGAGTCCGCTTTTGCGTTTTGTGAAAGCAACTGCTCTCACGTTCTTATCTTCGATTC
TCTTCATCTCTATCTTTCTTTTTGTGCCTCCCATATTGAAACTTCAAAGGAAGAAAGCCA
AATTACAGACAGAGATTGTTTGAAACTTGTAGAAAGCTAATTACACAGAAAGACAGAGAT
TGTTTTCATCGTCCTCTCTTTATACTAGTGTACTCTCTTCCCATATCTCAACTTTTTACA
TTATTGATTCATTCTCTATTAAGAAAATTTCCATAATGGGTTGTTTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 17-K119267-0022-181-H05C-8409
GenBank Accession LN494085 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K119267-0022-181-H05C-8409
CATTTACATTGAAACAACCCATTATGGAAATTTTCTTAATAGAGAATGAATCAATAATGT
AAAAAGTTGAGATATGGGAAGAGAGTACACTAGTATAAAGAGAGGACGATGAAAACAATC
TCTGTCTTTCTGTGTAATTAGCTTTCTACAAGTTTCAAACAATCTCTGTCTGTAATTTGG
CTTTCTTCCTTTGAAGTTTCAATATGGGAGGCACAAAAAGAAAGATAGAGATGAAGAGAA
TCGAAGATAAGAACGTGAGAGCAGTTGCTTTCACAAAACGCAAAAGCGGACTCTTCCACA
AAGCTTCAGAGCTCTGTCTCCTCTCTCCGGGGACTCAAATCGCAATCTTAGCAACTCCTC
TTTCTTCCCACTCCCATGCTTCTTTCTACTCTTTCGGTCATTCCTCTGTCGATCACGTTG
TCTCCTCTCTCCTTCACAATCAGCATCCATCTCTTCCCACAAACCAAGATAACAGATCA

Last Updated on 06.12.2018 16:11