Sequences from confirmation amplicon


Confirmation sequences for line 182B12 and hit At3g02150 are shown.
Line ID 182B12
Locus At3g02150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:391724
Primers used for PCR PC06 - 8474, show primer details
   
Sequence Name 19-K019978-022-182-B12C-PC06
GenBank Accession LN494566 [GenBank]
Sequencing primer gene specific primer, PC06: 5'-ATGTTCCAGGTTGTGATAAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K019978-022-182-B12C-PC06
CCAACATGATGATTGTTTGTGTAAACGACATCGTTTTCTCCTTTCTCTCTATCGTTTTCT
TCGCGGCTAGTAGTAGTAGACTTTTGTACACATCCATTGATTTTAAACCCGAGTTGGGTC
GGATCTTGACCGGGCCGTTGACCAAGATTCAAGAAGGACTGATGATGGTTGAAGATGCTG
AAATTTTCCGGCGAGATAGGTAACGGAGGTAGCTCGTCGATCTCCTCTTTAGCTGCATCA
AGCAACCAGTCAACGGCTTTGCTAGGCTGGTCAACACCGAGCCGTTCTTGAAGATCGTAG
AGCTGAATAGCCGTTGGGACTGATAATCTCACGCGTCTGTCACGTAGTCCACGTAACGTA
CACACTTTGCTGTGACGGTCTTTGCCTCCAAAGGCGCGTGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATTTCGACGTGTCTACATTCA
   
Sequence Name 23-K019978-022-182-B12C-8409
GenBank Accession LN496370 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K019978-022-182-B12C-8409
TAGATTTCACGCGCCTTTGGAGGCAAAGACCGTCACAGCAAAGTGTGTACGTTACGTGGA
CTACGTGACAGACGCGTGAGATTATCAGTCCCAACGGCTATTCAGCTCTACGATCTTCAA
GAACGGCTCGGTGTTGACCAGCCTAGCAAAGCCGTTGACTGGTTGCTTGATGCAGCTAAA
GAGGAGATCGACGAGCTACCTCCGTTACCTATCTCGCCGGAAAATTTCAGCATCTTCAAC
CATCATCAGTCCTTCTTGAATCTTGGTCAACGGCCCGGTCAAGATCCGACCCAACTCGGG
TTTAAAATCAATGGATGTGTACAAAAGTCTACTACTACTAGCCGCGAAGAAAACGATAGA
GAGAAAGGAGAAAACGATGTCGTTTACACAAACAATCATCATGTTGGGTCTTATGGAACT
TATCACAACCGGGAAC

Last Updated on 06.12.2018 16:11