Sequences from confirmation amplicon


Confirmation sequences for line 183F05 and hit At1g75430 are shown.
Line ID 183F05
Locus At1g75430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28308631
Primers used for PCR JU14 - 8474, show primer details
   
Sequence Name 11-K021056-022-183-F05C-8409
GenBank Accession LN490917 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K021056-022-183-F05C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATGGGATCATCAGCT
TGTTCATCATCACTTCAGCGACTTGGTTTGGTTCAAGGACCACAGAGACATGCTTGGAAA
CCCATCAGAGGCTTGCCTGAGACTTCCGTTGCAATTCTCCGAGCTTGGCTCTTTCAACAT
TTCTTGCACCCGTCAGTTTTCTTGTTGCTCAAGTGTAATAACACTAGTGATGAATCAAAG
TTAAAATGTTGGAGAAATAACATTAGTTTGATTGCTGTAATGAAAACAGTTATCCGAATG
AAGCAGAGAAGCTGGTGTTGGCGTCTCAAACAGGACTTTCGAAGAACCAAGTACACTCCT
CTGAATCTAATTCTGCTTTTTTTCTGAAATTTTTGTTATCAAGAAGATTCTGACTAAGAT
CATTTTTGTTGTTGATGATTAAAGGTATCAAATTGGTTTATAAATGCTCGAGTTCGACTC
TGGAAACCGA
   
Sequence Name 12-K021056-022-183-F05C-JU14
GenBank Accession LN491377 [GenBank]
Sequencing primer gene specific primer, JU14: 5'-ATCTCTTCTATCATCGGTTTCCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K021056-022-183-F05C-JU14
CGAACTCGAGCATTTATAAACCAATTTGATACCTTTAATCATCAACAACAAAAATGATCT
TAGTCAGAATCTTCTTGATAACAAAAATTTCAGAAAAAAAGCAGAATTAGATTCAGAGGA
GTGTACTTGGTTCTTCGAAAGTCCTGTTTGAGACGCCAACACCAGCTTCTCTGCTTCATT
CGGATAACTGTTTTCATTACAGCAATCAAACTAATGTTATTTCTCCAACATTTTAACTTT
GATTCATCACTAGTGTTATTACACTTGAGCAACAAGAAAACTGACGGGTGCAAGAAATGT
TGAAAGAGCCAAGCTCGGAGAATTGCAACGGAAGTCTCAGGCAAGCCTCTGATGGGTTTC
CAAGCATGTCTCTGTGGTCCTTGAACCAAACCAAGTCGCTGAAGTGATGATGAACAAGCT
GATGATCCCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAG
Primers used for PCR JU13 - 8474, show primer details
   
Sequence Name 13-K021056-022-183-F05C-oriL
GenBank Accession LN491840 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K021056-022-183-F05C-oriL
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAACAAGCTGAGCTGTGATAAGCC
AGAGCTGATGATCTTGGGAACATCTTGATGAGAAATGATGAATCTCCGACGAACAGAGTT
TAGCTGAGAGATTATTGCTTCTTCAAGACTACCGAAATGTCGAGTCATGGCTTGGAGAGC
TAAACCGGTGTACACTTTAGATGATCCTTCTCCTGCTATTTCCTCAAATGATGAAATCAC
TTGCTCCAGTTGATTGCAGTACTGCTCAAATCTTTCTTCTACCTGTCCATGAACCGGATT
TAACCAAAATTATGTTGAAACGAAAACCAAAGGAAA
   
Sequence Name 14-K021056-022-183-F05C-JU13
GenBank Accession LN492309 [GenBank]
Sequencing primer gene specific primer, JU13: 5'-ATGCAATATGTTTTCCTTTGGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K021056-022-183-F05C-JU13
CGTTCAACATAATTTTGGTTAAATCCGGTTCATGGACAGGTAGAAGAAAGATTTGAGCAG
TACTGCAATCAACTGGAGCAAGTGATTTCATCATTTGAGGAAATAGCAGGAGAAGGATCA
TCTAAAGTGTACACCGGTTTAGCTCTCCAAGCCATGACTCGACATTTCGGTAGTCTTGAA
GAAGCAATAATCTCTCAGCTAAACTCTGTTCGTCGGAGATTCATCATTTCTCATCAAGAT
GTTCCCAAGATCATCAGCTCTGGCTTATCACAGCTCAGCTTGTTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAA

Last Updated on 06.12.2018 16:11