Sequences from confirmation amplicon


Confirmation sequences for line 183F08 and hit At3g20550 are shown.
Line ID 183F08
Locus At3g20550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7177601
Primers used for PCR BL66 - 8474, show primer details
   
Sequence Name 28-K105138-0022-183-F08C-BL66
GenBank Accession LN498842 [GenBank]
Sequencing primer gene specific primer, BL66: 5'-TAAATGATTCTTCTCGTTTTGGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K105138-0022-183-F08C-BL66
TATTCCTCTCTCCTCTTCTCTCCGCCGAAGTAGTCACAGTTCCGGCCACCAGGTAAAATT
TTTCTTTCTCGTTATGCAGCGATTTTTTCTCTTAACGATTCTTGCAACCCTAGACTATGC
CCGGAACTTCTGAATTTAGTATTTGAAATCTGTAGCTTACGTTTTTTGGTTTAGGGCTTT
TGCTCCATTTCTAGTTTCTATGCATTTGGGAATTAGGGTTTACTGTGTTATCTGGAGCTC
ACATGCCTTTTCCTTCTGACCCTGGTTTCAATTTGTGATCTTTCCAAAGGCAAAGAAGAG
AGAAAACGACCCTGACTCCAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 12-K105139-0022-183-F08C-8409
GenBank Accession LN491572 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K105139-0022-183-F08C-8409
TACATTGAATATTGGAGTCAGGGTCGTTTTCTCTCTTCTTTGCCTTTGGAAAGATCACAA
ATTGAAACCAGGGTCAGAAGGAAAAGGCATGTGAGCTCCAGATAACACAGTAAACCCTAA
TTCCCAAATGCATAGAAACTAGAAATGGAGCAAAAGCCCTAAACCAAAAAACGTAAGCTA
CAGATTTCAAATACTAAATTCAGAAGTTCCGGGCATAGTCTAGGGTTGCAAGAATCGTTA
AGAGAAAAAATCGCTGCATAACGAGAAAGAAAAATTTTACCTGGTGGCCGGAACTGTGAC
TACTTCGGCGGAGAGAAGAGGAGAGAGTGAATAATAATAATCGCAGAGATAAAAAGAATC
AGAGCCAAAACGAGAGAAATTCATTT

Last Updated on 06.12.2018 16:11