Sequences from confirmation amplicon


Confirmation sequences for line 184F05 and hit At3g22110 are shown.
Line ID 184F05
Locus At3g22110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7792810
Primers used for PCR KT27 - 8474, show primer details
   
Sequence Name 44-K100814-0022-184-F05C-8409
GenBank Accession LN505107 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K100814-0022-184-F05C-8409
CTTTACAATTGAATATATCCTGCGGATATCATTGTTTTATCTTTCTTTTGGGCTTAAGAT
GTTTCAGCAGCGGGTTGAGTCACACCGTGCTTGACCAACAGTTTGGTGAGCGACTCAGGT
GAGTGAACATGGTACTTAACAGTCTTGGATGGAGTCAGATACACTTCAGCAAGCTCTAGC
TTCTCAGATGTTAAGCTAGTGCTGTCCATTGTCTTGGTCAGAACCTTCAAGGCAAGCTCG
ACTGCTTCTTCCCTGGTTGCATCGTCCTTGTAGTCTTGTTTCAGGATAGACTGAGCCGCC
TGATTGTTTGCTCCAACAGCTGCAGCTTTCCAACCACCATAGTTCCCACTCGGGTCACTC
ATATAAAGCTGGAAACCATGGTGCTTGTCCCATCCTGCAAAGAGAAAGGAGACACCAAAC
GGGCGAAGACCACCAAACTGTGTGTATCCTTGTTTTGTGTCACAAAGAGATTGAACCAGC
TGCTCAACCGGCATGGGCTCTTGGTACATGAAGGTGTAACGTTGAGCTTGAACTCGAGCC
GTGTTGATNAGTATGTTAGCAA
   
Sequence Name 44-K100826-0022-184-F05C-KT27
GenBank Accession LN505109 [GenBank]
Sequencing primer gene specific primer, KT27: 5'-TGCTAACATACTTATCAACACGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K100826-0022-184-F05C-KT27
GCCCATGCCGGTTGAGCAGCTGGTTCAATCTCTTTGTGACACAAAACAAGGATACACACA
GNTTTGGTGGTCTTCGCCCGTTTGGTGTCTCCTTTCTCTTTGCAGGATGGGACAAGCACC
ATGGTTTCCAGCTTTATATGAGTGACCCGAGTGGGAACTATGGTGGTTGGAAAGCTGCAG
CTGTTGGAGCAAACAATCAGGCGGCTCAGTCTATCCTGAAACAAGACTACAAGGACGATG
CAACCAGGGAAGAAGCAGTCGAGCTTGCCTTGAAGGTTCTGACCAAGACAATGGACAGCA
CTAGCTTAACATCTGAGAAGCTAGAGCTTGCTGAAGTGTATCTGACTCCATCCAAGACTG
TTAAGTACCATGTTCACTCACCTGAGTCGCTCACCAAACTGTTGGTCAAGCACGGTGTGA
CTCAACCCGCTGCTGAAACATCTNAAGCCCAAAAGAAAGATAAAACAATGATTTCCGCAG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATG
TAGATGTCCGC

Last Updated on 06.12.2018 16:11