Sequences from confirmation amplicon


Confirmation sequences for line 185B11 and hit At2g34810 are shown.
Line ID 185B11
Locus At2g34810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14686603
Primers used for PCR AD01 - 8474, show primer details
   
Sequence Name 05-K026796-022-185-B11C-8409
GenBank Accession LN488205 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K026796-022-185-B11C-8409
CCATGTACATTTCCCGGACATGAAGCCATTTACAATTGTAAACATTACCACCTCTATGCA
CAAACGCCGTCGCATCAGACGCTATCTCAGCCATTTTCCCTCCCCATGGATTCCATTGCA
TCCAAACTCGTCGTGGAAGTGGCGAATGATCAATCATTGTTTGAAAAATCTTTGCAAGAC
CCTGTTTTGAGATTGGTGCTTGAACAAAATCTGAAGTACGTTTCCAGTAGTATTCGTTGG
TTGATACTCTTTTGAGAAGTAACTCTATCGGTGCTTTTTCTGGATAATTCGCCCAGAAAA
CACTAGATTCGATCCATTTCTTGACCATACAATCTTCTTTCGTTAAATGTAGCTCAGGGA
AACTCTGTTTCGTTATGTTTAAAAGTCTCTCAGGTAAACCAAGAAACATTCCGATGAAGG
AGACTTTAATCGTCTTACCTTTTGAGATTTTCCGGTTTATTGGTTTAATCTCTAACCGGA
TAAACAAATCTCTGTCGATAACCGGAGCTACTTGTTGCCATTTATGAACTAAATCAACGG
CTCCTTCTCTCACCGTTCTTTCTAGTTTAAAAACTGTGACTTTCTCCGGAACCCTAACGA
GTTTTATCTTCC
   
Sequence Name 06-K026796-022-185-B11C-AD01
GenBank Accession LN488656 [GenBank]
Sequencing primer gene specific primer, AD01: 5'-AGTCATTCTTGCCTGGAAGATAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K026796-022-185-B11C-AD01
GGGTTCGGAGAAAGTCACAGTTTTTAAACTAGAAAGAACGGTGAGAGAAGGAGCCGTTGA
TTTAGTTCATAAATGGCAACAAGTAGCTCCGGTTATCGACAGAGATTTGTTTATCCGGTT
AGAGATTAAACCAATAAACCGGAAAATCTCAAAAGGTAAGACGATTAAAGTCTCCTTCAT
CGGAATGTTTCTTGGTTTACCTGAGAGACTTTTAAACATAACGAAACAGAGTTTCCCTGA
GCTACATTTAACGAAAGAAGATTGTATGGTCAAGAAATGGATCGAATCTAGTGTTTTCTG
GGCGAATTATCCAGAAAAAGCACCGATAGAGTTACTTCTCAAAAGAGTATCAACCAACGA
ATACTACTGGAAACGTACTTCAGATTTTGTTCAAGCACCAATCTCAAAACAGGGTCTTGC
AAAGATTTTTCAAACAATGATTGATCATTCGCCACTTCCACGACGAGTTTGGATGCAATG
GAATCCATGGGGAGGGAAAATGGCTGAGATAGCGTCTGATGCGACGGCGTTTGTGCATAG
AGGTGGTAATGTTTACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA

Last Updated on 06.12.2018 16:11