Sequences from confirmation amplicon


Confirmation sequences for line 185F12 and hit At1g20925 are shown.
Line ID 185F12
Locus At1g20925 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7291714
Primers used for PCR PW05 - 8474, show primer details
   
Sequence Name 17-K024603-022-185-F12C-8409
GenBank Accession LN493697 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K024603-022-185-F12C-8409
AATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTTAGCCAACTTACTGATT
TTGAGAGAATGTATATGTGACAAGGTTGGGACTTGGGGCAAGGTCAAACAAAGAGTGTGC
TCTGTAGCAGAGAAAATCAATCTACGAACAATATTTGCTCCTTCAACTATTGCAGCGGTT
TGTTTCATGTAGATTTCATCTAGTTAAGCAACTAATCCTCTTTCTCTTCAGCTTATCGCG
CTTGCGGTTGGGTTAAATCCTTTACTACGGAAGCTACTAGTCGGTAACACAGCTCCACTT
CGAGTGATTGAGGACTCAGTATCTCTATTGGGGTATTCTACAACATATTAAAAATCTCAC
TTGTCAGTGATAATTAACAATGCTTACTTGAGTAGCTTAATTTTCTTGAAGGGACGGGGC
GATTCCTGTTCTGACTCTGATTGTTGGAGGAAACCTTCTCAATGGTAGATAAACAATAAC
AAATGGCTGCAGAAACTTTTTTTCCTAAGACAAAACCTTAAATCTCTGTTCTCTCTCTTA
TAATAATCACAGGTCT
   
Sequence Name 18-K024603-022-185-F12C-PW05
GenBank Accession LN494145 [GenBank]
Sequencing primer gene specific primer, PW05: 5'-TATTCCTGAACCTCTCAGACCTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K024603-022-185-F12C-PW05
ATTATTATAAGAGAGAGAACAGAGATTTAAGGTTTTGTCTTAGGAAAAAAAGTTTCTGCA
GCCATTTGTTATTGTTTATCTACCATTGAGAAGGTTTCCTCCAACAATCAGAGTCAGAAC
AGGAATCGCCCCGTCCCTTCAAGAAAATTAAGCTACTCAAGTAAGCATTGTTAATTATCA
CTGACAAGTGAGATTTTTAATATGTTGTAGAATACCCCAATAGAGATACTGAGTCCTCAA
TCACTCGAAGTGGAGCTGTGTTACCGACTAGTAGCTTCCGTAGTAAAGGATTTAACCCAA
CCGCAAGCGCGATAAGCTGAAGAGAAAGAGGATTAGTTGCTTAACTAGATGAAATCTACA
TGAAACAAACCGCTGCAATAGTTGAAGGAGCAAATATTGTTCGTAGATTGATTTTCTCTG
CTACAGAGCACACTCTTTGTTTGACCTTGCCCCAAGTCCCAACCTTGTCACATATACATT
CTCTCAAAATCAGTAAGTTGGCTAACAGGATATATTCAATTGTANAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTT
Primers used for PCR PW06 - 8474, show primer details
   
Sequence Name 33-K024604-022-185-F12C-8409
GenBank Accession LN500840 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K024604-022-185-F12C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAAACAAACCGCTGCAATAGTTGAAGG
AGCAAATATTGTTCGTAGATTGATTTTCTCTGCTACAGAGCACACTCTTTGTTTGACCTT
GCCCCAAGTCCCAACCTTGTCACATATACATTCTCTCAAAATCAGTAAGTTGGCTAACAA
GAATATAAGAAGGAAAAGTTTAGACTAATACCTGTTCCGCAACTTCGACTTTGGGAGAAA
TCAGTGGCATTGTAGACGAGGTACTGTTTATTGCGGTTTCTCCAGCAGGATTTGCTAGCA
TCCGCATAAGATTGTATACATAAGTCCATATGTAAATGGCTCCAATCTGATATACATCAC
AAAACGTTTTTGACTTGTATTGGCATTAAAAAAAAACTTTAGTAGAGAAGCATAAGAAGT
AGTTAAGAAATG
   
Sequence Name 34-K024604-022-185-F12C-PW06
GenBank Accession LN501262 [GenBank]
Sequencing primer gene specific primer, PW06: 5'-GCGGTATGTCACCATTTCTTAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K024604-022-185-F12C-PW06
ACTTCTTATGCTTCTCTACTAAAGTTTTTTTTTAATGCCAATACAAGTCAAAAACGTTTT
GTGATGTATATCAGATTGGAGCCATTTACATATGGACTTATGTATACAATCTTATGCGGA
TGCTAGCAAATCCTGCTGGAGAAACCGCAATAAACAGTACCTCGTCTACAATGCCACTGA
TTTCTCCCAAAGTCGAAGTTGCGGAACAGGTATTAGTCTAAACTTTTCCTTCTTATATTC
TTGTTAGCCAACTTACTGATTTTGAGAGAATGTATATGTGACAAGGTTGGGACTTGGGGC
AAGGTCAAACAAAGAGTGTGCTCTGTAGCAGAGAAAATCAATCTACGAACAATATTTGCT
CCTTCAACTATTGCAGCGGTTTGTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11