Sequences from confirmation amplicon


Confirmation sequences for line 186D09 and hit At3g18880 are shown.
Line ID 186D09
Locus At3g18880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6509724
Primers used for PCR KT25 - 8474, show primer details
   
Sequence Name 31-K100811-0022-186-D09C-8409
GenBank Accession LN500075 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K100811-0022-186-D09C-8409
GCCATTTACATTGAATATATCATATGAAAATTGAATTGCATTTTCCTTGAAAATCACTTT
TCCTTGAAATGCAATTCAACTCTTAGGACTCCTGTAACATCGGCGATCGAGTATTTTCCC
CTTCATTTTTTGAATTTGATATACATTTGATCAAGAGCTCGAAAATTTAGGGAAGCTCTA
GGAATATTTGAACTCAACCATTGGAAGTAGACTTGCCTGTTTGTTATTAGGCTGCTTTGG
AAATGAAACTTGATTTGGTTGTTTGAAATATTAGGTGAAATTGGATCCTTCAAGGCCTTT
GAGCAAGCATAAGCATTGGATTGTTTCAGAAATCATAAAGAAAGCACGCATTTATTCTCC
ACAAGCTGCTGCTGCGGCTCTCAATTCCTCAACCTCCATTGCTGAGCAGCCCCCTGTTTC
GTCTTCCTAAGGTGATTTAGTTATTGCTCTCTAATTTTTTGTATCAGAATATTTTGTGTC
ACAGTATTGGCCTTTGATACCTTTAGTGGAACTGCA
   
Sequence Name 31-K100825-0022-186-D09C-KT25
GenBank Accession LN500077 [GenBank]
Sequencing primer gene specific primer, KT25: 5'-CAATTCTCATGCAGTTCCACTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K100825-0022-186-D09C-KT25
TTCTGATCAAAAAATTAGAGAGCAATAACTAAATCACCTTAGGAAGACGAAACAGGGGGC
TGCTCAGCAATGGAGGTTGAGGAATTGAGAGCCGCAGCAGCAGCTTGTGGAGAATAAATG
CGTGCTTTCTTTATGATTTCTGAAACAATCCAATGCTTATGCTTGCTCAAAGGCCTTGAA
GGATCCAATTTCACCTAATATTTCAAACAACCAAATCAAGTTTCATTTCCAAAGCAGCCT
AATAACAAACAGGCAAGTCTACTTCCAATGGTTGAGTTCAAATATTCCTAGAGCTTCCCT
AAATTTTCGAGCTCTTGATCAAATGTATATCAAATTCAAAAAATGAAGGGGAAAATACTC
GATCGCCGATGTTACAGGAGTCCTAAGAGTTGAATTGCATTTCAAGGAAAAGTGATTTTC
AAGGAAAATGCAATTCAATTTTCATATGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTC

Last Updated on 06.12.2018 16:11