Sequences from confirmation amplicon


Confirmation sequences for line 186F12 and hit At4g15500 are shown.
Line ID 186F12
Locus At4g15500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8858690
Primers used for PCR RE38 - 8474, show primer details
   
Sequence Name 11-K025944-022-186-F12C-8409
GenBank Accession LN490951 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K025944-022-186-F12C-8409
GTAGATTTCCCGGACATGAAGCCATTTACATTTTGTATTTTATGATATTTTTCTACATTT
ATTATCAATATTTGTATAAAAAAATAGCACGCCGTGGAAAGGCCTCTCTACCCAACCTTC
CATAAATCGAGGTATCTAAATTGGAAATATTGTAAAGAAGACAAAAACTTCTTTTTGAGT
TTTACCAACAAAAAAAAAAATATGGAGATGGAATCGTCGTTACCTCATGTGATGCTCGTA
TCATTCCCAGGGCAAGGTCACATAAGCCCTCTTCTTCGTCTCGGAAAGATCATTGCCTCT
AAAGGCTTAATCGTCACCTTTGTAACCACAGAGGAACCATTGGGCAAGAAGATGCGTCAA
GCCAACAATATTCAAGACGGTGTGCTCAAACCGGTCGGGCTAGGTTTTCTCCGGTTCGAG
TTCTTCGAGGATGGATTTGTCTACAAAGAAAACTTTGATTTGTTACAAAAATCACTTGAA
GTTTCCGGAAAACGAGAGATCAAGAATCTTGTCAAGAAATATGAGAAGCAACCAGTGAGA
TGTCTCATAAATAATGCCTTTGTTCCATGGGTTTGTGACATAGCCGAGGAGCTTCAAATC
CCATCAGCTGTTCTTTGGGTCCAGTCTTGTGCTTGCC
   
Sequence Name 12-K025944-022-186-F12C-RE38
GenBank Accession LN491410 [GenBank]
Sequencing primer gene specific primer, RE38: 5'-GGTGGTGGTAATAGTAATAAGCGG-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K025944-022-186-F12C-RE38
GGCAAGCACAAGACTGGACCCAAAGAACAGCTGATGGGATTTGAAGCTCCTCGGCTATGT
CACAAACCCATGGAACAAAGGCATTATTTATGAGACATCTCACTGGTTGCTTCTCATATT
TCTTGACAAGATTCTTGATCTCTCGTTTTCCGGAAACTTCAAGTGATTTTTGTAACAAAT
CAAAGTCTTCTTTGTAGACAAATCCATCCTCGAAGAACTCGAACCGGAGAAAACCTAGCC
CGACCGGTTTGAGCACACCGTCTTGAATATTGTTGGCTTGACGCATCTTCTTGCCCAATG
GTTCCTCTGTGGTTACAAAGGTGACGATTAAGCCTTTAGAGGCAATGATCTTTCCGAGAC
GAAGAAGAGGGCTTATGTGACCTTGCCCTGGGAATGATACGAGCATCACATGAGGTAACG
ACGATTCCATCTCCATATTTTTTTTTTTGTTGGTAAAACTCAAAAAGAAGTTTTTGTCTT
CTTTACAATATTTCCAATTTAAATACCTCGATTTATGGAAGGTTGGGTAGAGAGGCCTTT
CCACGGCGTGCTATTTTTTTATACAAATATTGATAATAAATGTAGAAAAATATCATAAAA
TACAAAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA

Last Updated on 06.12.2018 16:11