Sequences from confirmation amplicon


Confirmation sequences for line 187H05 and hit At5g07420 are shown.
Line ID 187H05
Locus At5g07420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2349942
Primers used for PCR KT23 - 8474, show primer details
   
Sequence Name 47-K100814-0022-187-H05C-8409
GenBank Accession LN506131 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K100814-0022-187-H05C-8409
TTACAATTGAATATGAGAATAAATTGATTATGGTTTTACTTTTTTTTTTTTATCCCAGCA
AATACAACTATACTGAAATTTAAGTACAACGGTTTAAAGTATTTTAATTTGTGCAATTTT
TATCAGGTTTCTTTGTCTCAAAAACGAAAACTGGATTCAATACATTAATCAATCTTATAG
GTATGATAAAATTGTGATTTACTTACAATGATAACACGTCGCGATGCTTCTGCAGCTTCA
AGTTCGGGGTCTAGCGTGCCCCGTCTCTGGGAAAAGGGTTTAACGTTGGCCATGAACCAT
TGTGGGATTTGGGCTCTGTCTGCCGGAATTGGGGTTATATCGTCGGCAAGAACCACCGGC
GAGGCAAAAAATACAAGTAATGCAACTAGGGCTAGAGATATGTAACCCATCTTTATAAAT
TTATTAATTTTTAAGTTTTTTTTTAAGGTAAAAGAAAATAAAAGAAAAAAAGATTTTTTT
TAATTTGGGAATAAAAATGGAAAAAA
   
Sequence Name 47-K100826-0022-187-H05C-KT23
GenBank Accession LN506133 [GenBank]
Sequencing primer gene specific primer, KT23: 5'-CATCTCTTCCATTTTTATTCCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K100826-0022-187-H05C-KT23
TTATTTTCTTTACTTTAAAAAAAACTTAAAAATTAATAAATTAATAAAGATGGGTTACAT
ATCTCTAGCCCTAGTTGCATTACTTGTATTTTTTGCCTCGCCGGTGGTTCTCGCCGACGA
TATAACCCCAATTCCGGCAGACAGAGCCCAAATCCCACAATGGTTCATGGCCAACGTTAA
ACCCTTTTCCCAGAGACGGGGCACGCTAGACCCCGAACTTGAAGCTGCAGAAGCATCGCG
ACGTGTCATCATCGTAAGTAAATCACAATTTTATCATACCTATNAGATTGATTAATGTAT
TGAATCCAGTTTTCGTTTTTGAGACAAAGAAACCTGATAAAAATTGCACAAATTAAAATA
CTTTAAACCGTTGTACTTAAATTTCAGTATAGTTGTATTTGCTGGGATAAAAAAAAAAAA
GTAAAACCATAATCAATTTATTCTCATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACTTGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATTTCC

Last Updated on 06.12.2018 16:11