Sequences from confirmation amplicon


Confirmation sequences for line 189F10 and hit At5g42910 are shown.
Line ID 189F10
Locus At5g42910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17204554
Primers used for PCR MJ07 - 8474, show primer details
   
Sequence Name 55-K017462-022-189-F10C-MJ07
GenBank Accession LN508654 [GenBank]
Sequencing primer gene specific primer, MJ07: 5'-AGCAAAAATGAGCAGTTTGGTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K017462-022-189-F10C-MJ07
AGATGGATAGATAATATATATATATATATATAACATTTTCTTACTTGCTTTCGAGCCCGT
GATCTCGCTGCGGATTCTCGGTTCTTAATCTTTCTCCTAAGCTTTTTGTCAACGAATTGT
TTTTCCGCGGTAATTTCACTATTAATCTTCCCACCTCTTGTACTAGTACTACCATTACTA
ATGTATGGAGATGGTGAGAGTAATGAAGACTCACCGTTAATCTTTTGTTTCCCATTTAAT
GTTGGAAATGGGGTAACCGGTGAACATGTTATGGCTTCACTTGGAATTGTCGCAACACTT
GGGACCAAGCTTCTCTTCGTATCTTGGAGACTTTGGTTACCAGCAATGAAATAGTTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGT
   
Sequence Name 56-K017462-022-189-F10C-8409
GenBank Accession LN508966 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K017462-022-189-F10C-8409
TTTACATTGAACTATTTCATTGCTGGTAACCAAAGTCTCCAAGATACGAAGAGAAGCTTG
GTCCCAAGTGTTGCGACAATTCCAAGTGAAGCCATAACATGTTCACCGGTTACCCCATTT
CCAACATTAAATGGGAAACAAAAGATTAACGGTGAGTCTTCATTACTCTCACCATCTCCA
TACATTAGTAATGGTAGTACTAGTACAAGAGGTGGGAAGATTAATAGTGAAATTACCGCG
GAAAAACAATTCGTTGACAAAAAGCTTAGGAGAAAGATTAAGAACCGAGAATCCGCAGCG
AGATCACGGGCTCGAAAGCAAGTAAGAAAATGTTATATATATATATATATATTATCTATC
CATCTTGATTGATTGTGTTTTATAAGTACCAAACT

Last Updated on 06.12.2018 16:11