Sequences from confirmation amplicon


Confirmation sequences for line 190B06 and hit At4g10960 are shown.
Line ID 190B06
Locus At4g10960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6718731
Primers used for PCR MP04 - 8474, show primer details
   
Sequence Name 30-K019216-022-190-B06C-8409
GenBank Accession LN499528 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K019216-022-190-B06C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATGAAGCCATATGTAG
ATTTCTCCATATTCCACCACGTGGCATTTTCATTATTCCATTCCCTTAATTGAAAACGTA
ATAATACATGCGTTTTTAAATTAAGCCTAAGTTTAATGTAACCATATTATTCACGTAACC
ATCACCGATTCTATATATTAAAAACCTCCAAGCACTCTTTCGCTGGAGATCACAAAGCAA
TCTAAAAACATTTCTCTTGATCTTTTTCCTTTACCAAAAAACAAAGATCCTTTCTTCTGT
TTCGTAATGATGGCTAGAAACGTTCTAGTAAGCGGTGGTGCTGGATACATCGGTAGTCAC
ACGGTGCTTCAACTTCTTCTCGGTGGTTACTCCGTCGTAGTTGTTGATAATCTCGATAAT
TCCTCCGCCGTCTCTCTTCAACGTGTGAAGAAACTCGCGGCTGAACACGGAGAACGCCTC
TCCTTCCACCAGGTTCGGTGAAATTTCGCTTTCTTAGGGCTAGGAAACGTGTGATTGAAG
GATTTGTGTTTGTGTAGTTGATGATTTCAACGAATTTGGGATTATGATTTGACTTGTTAA
TCATTGATTTGACTACGAGGCTCTTTTTTTTGGGTTTATTTATTTATTTAATTTTG
   
Sequence Name 31-K019216-022-190-B06C-MP04
GenBank Accession LN499959 [GenBank]
Sequencing primer gene specific primer, MP04: 5'-CGAGAATTTATGCCCAAAATTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K019216-022-190-B06C-MP04
TAAATAAATAAACACAAAAAAAAGAGCCTCGTAGTCAAATCAATGATTAACAAGTCAAAT
CATAATCCCAAATTCGTTGAAATCATCAACTACACAAACACAAATCCTTCAATCACACGT
TTCCTAGCCCTAAGAAAGCGAAATTTCACCGAACCTGGTGGAAGGAGAGGCGTTCTCCGT
GTTCAGCCGCGAGTTTCTTCACACGTTGAAGAGAGACGGCGGAGGAATTATCGAGATTAT
CAACAACTACGACGGAGTAACCACCGAGAAGAAGTTGAAGCACCGTGTGACTACCGATGT
ATCCAGCACCACCGCTTACTAGAACGTTTCTAGCCATCATTACGAAACAGAAGAAAGGAT
CTTTGTTTTTTGGTAAAGGAAAAAGATCAAGAGAAATGTTTTTAGATTGCTTTGTGATCT
CCAGCGAAAGAGTGCTTGGAGGTTTTTAATATATAGAATCGGTGATGGTTACGTGAATAA
TATGGTTACATTAAACTTAGGCTTAATTTAAAAACGCATGTATTATTACGTTTTCAATTA
AGGGAATGGAATAATGAAAATGCCACGTGGTGGAATATGGAGAAATCTACATATGGCTTC
ATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGA

Last Updated on 06.12.2018 16:11