Sequences from confirmation amplicon


Confirmation sequences for line 190D07 and hit At2g02560 are shown.
Line ID 190D07
Locus At2g02560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:694864
Primers used for PCR FA32 - 8474, show primer details
   
Sequence Name 73-K037325-022-190-D07C-8409
GenBank Accession LN512846 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K037325-022-190-D07C-8409
CCGGACTGAGCCATTTCAATTGAATATATCCTTCCTTCTTGTGGAGCTTTCATCTCTGAT
AAGGTTTGTTTGCTTCTCGAACAAATTTCCCCTGATTTTTGTTGTTTCTACTTTCTAGGT
TGGTATATATATTAAAAGATGACTTCGTCTGTGTTTTTTCAGTGTTTCAGACCTGCACAT
GACAGCTCTTGCACTCGAACTCTGCTGCACTCTGATGACTGGAAAGAGTTGTAGTGAAAA
TATCAGTTTGGCGGTCCGCAACAAAGTTCTTCCGCAGGCACTAACTTTAGTTAAAAGCCC
ATTGCTCCAGGGTCAAGCACTTTTGGTAAATTTTTTGTTACTGATGGAATTGTTATATAC
TTTGCCTGACCTTAAAGATTAGAAGGCAATGATTTCTTGGATGTTTGTCACAGGATCTGC
AAAAATTCTTTGAAGCTCTGGTGTATCATGCAAATACGAGTTTCTACACCTGCCTGGAAT
CA
   
Sequence Name 65-K037325-022-190-D07C-FA32
GenBank Accession LN511340 [GenBank]
Sequencing primer gene specific primer, FA32: 5'-GATTCCAGCAAGGTGTAGAAACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K037325-022-190-D07C-FA32
TTCAAGAATTTTTGCAGATCCTGTGACAAACATCCAAGAAATCATTGCCTTCTAATCTTT
AAGGTCAGGCAAAGTATATAACAATTCCATCAGTAACAAAAAATTTACCAAAAGTGCTTG
ACCCTGGAGCAATGGGCTTTTAACTAAAGTTAGTGCCTGCGGAAGAACTTTGTTGCGGAC
CGCCAAACTGATATTTTCACTACAACTCTTTCCAGTCATCAGAGTGCAGCAGAGTTCGAG
TGCAAGAGCTGTCATGTGCAGGTCTGAAACACTGAAAAAACACAGACGAAGTCATCTTTT
AATATATATACCAACCTAGAAAGTAGAAACAACAAAAATCAGGGGAAATTTGTTCGAGAA
GCAAACAAACCTTATCAGAGATGAAAGCTCCACAAGAAGGAAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGG
CGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11