Sequences from confirmation amplicon


Confirmation sequences for line 191D05 and hit At2g36060 are shown.
Line ID 191D05
Locus At2g36060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15144155
Primers used for PCR AB02 - 8474, show primer details
   
Sequence Name 28-K021167-022-191-D05C-8409
GenBank Accession LN498659 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K021167-022-191-D05C-8409
TAGATTTCCCGGACATGAAGCCATGTAGAGTAATTCAACTGCCCCAAATTGCTCTCGACT
TTTTATTCTCGGATTAGCGTTTGTGCTTTCGCATCAGAGAATCTCTCAGTTCTTCTCAAT
TTAGGTCATCAGATCTCACAATCAATCGACTCGAACTCAAATCTCACTTCATCAATCAAA
TCATGACTCTTGGCTCAGGATCGAGTGTTGTCGGTAAATTTTCGATTTTTTTTTTTTTGG
GTCCTCTTTAAGTTTCCTAGGGTTTTTGATTTGACACGAAACGCAGCTTGGGTTTATCTT
AAATAATTTCAACCGATAATTTTCGGGATTTGTTTCTGGATTTTTCTGCATATGAGCCAA
ACTTGATATTTATGGATCTTTAACTCTGAAATGTTCGTCTTTTGATGTGAANCAAAAATG
TAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
TCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTT
CGACGTGTCTACATTCACG
   
Sequence Name 32-K021167-022-191-D05C-AB02
GenBank Accession LN500401 [GenBank]
Sequencing primer gene specific primer, AB02: 5'-CAATCAAAAGACGAACATTTCAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K021167-022-191-D05C-AB02
AAGATACATAAATATCAAGTATGGCTCATATGCAGAAAAATCCAGAAACAAATCACGAAA
ATTATCGGTTGAAATTATCTAAGATAAACCCAAGCTGCGTTTCGTGTCAAATCAAAAACC
CTAGGAAACTTAAAGAGGAACCAAAAAAAAAAAAATCAAAAATTTACCGACAACACTCGA
TCCTGAGCCAAGAGTCGTGATTTGATTGATGAAGTGAGATTTGACTCCAAGTCGATTGAT
TGGGAAATCTGATGACCTAAATTGAAAAAAACTGAAAGATTCTCTGATGCGAAAGCACAA
ACGCTAATCCGAAAATAAAAAGTCCAAAGCAATTTGGGGCAGTTGAATTACTCTACATGG
GTTCATGCCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCGCCGCGTTTTTA
TAAAATGAAAGTACTTTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAA
AGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGGGTCTACAT
Primers used for PCR AB01 - 8474, show primer details
   
Sequence Name 63-K113669-0022-191-D05C-AB01
GenBank Accession LN511032 [GenBank]
Sequencing primer gene specific primer, AB01: 5'-TATCACCACAACAGAACACACAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K113669-0022-191-D05C-AB01
AAGACGAACATTTCAGAGTTAAAGATACATAAATATCAAGTATGGCTCATATGCAGAAAA
ATCCAGAAACAAATCACGAAAATTATCGGTTGAAATTATCTAAGATAAACCCAAGCTGCG
TTTCGTGTCAAATCAAAAACCCTAGGAAACTTAAAGAGGAACCAAAAAAAAAAAATTCAA
AATTTTCCCGACAACCCTCATTCCGAACCAAAAATTCTGGATTTATTGGAGAAAGGGAAA
TTTGATTTCAATCCAATGGTTGGGAAAATCGAAGAACCAAATTGGAAAAAAATGGAAAAT
TTCTTTAAGGCAAAACCAAAACCCCTATCCCAAAAAAAAAAATTCAAAACCATTTGGGGG
AATTGAAATTACTCAACAGGGTTCCTGGCCGGGAAATTCTCCTGGGATCACCATGGATTA
GAAGGGCCAATTGGGAAAAAAAAAAAAATTATTTCCCATTTTTTTTTCATTTAAAAAAGG
AAAATTCCCCCACCTTTTTAAAAAA
   
Sequence Name 32-K113666-0022-191-D05C-8409
GenBank Accession LN500662 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K113666-0022-191-D05C-8409
AGTAATTCACTGCCCCAATTGCTCTCGACTTTTTATTCTCGGATTAGCGTTTGTGCTTTC
GCATCAGAGAATCTCTCAGTTCTTCTCAATTTAGGTCATCAGATCTCACAATCAATCGAC
TCGAACTCAAATCTCACTTCATCAATCAAATCATGACTCTTGGCTCAGGATCGAGTGTTG
TCGGTAAATTTTCGATTTTTTTTTTTTGGGTCCCCCTTAAATTTCCCAGGGGTTTTTAAT
TTAACCCAAAACCCACCTGGGGTTTATCTAAAAAAATTCCACCCAAAAATTTTCGGGATT
TGTTTCGGGATTTTCCGGCAAATAACCCAAACTGGATTTTTAGGTATCTTAAACCCTAAA
AGGTCCCCCTTTGGATGGAAATAAACAATGGGTTTTATAAAAATCCCCAAACTTGGGGGT
TCGGTTGGGGGAAAAA

Last Updated on 06.12.2018 16:11