Sequences from confirmation amplicon


Confirmation sequences for line 192F07 and hit At3g05260 are shown.
Line ID 192F07
Locus At3g05260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1498913
Primers used for PCR KT57 - 8474, show primer details
   
Sequence Name 78-K100822-0022-192-F07C-8409
GenBank Accession LN513645 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K100822-0022-192-F07C-8409
GCCATTTACAATTGCCATTTTCCTATTTTCCTTTCGAGTATTCGAAAATTTCCGGGAAAG
GTTTTGGTTATTGGCGACCGAGAAAATTACCGTAGAGATCGACAGAAACAAATAAGATAA
AGAAGGTGTCAAAAAGTGAGAGCTTCTTTGAGTGACGTGCCACGTTGCATCTCAATTTAG
ATGACGTGTACAAGCCATTGCAACGGTGCAGCCAGGGAGGAGTCCACGTGGGGAAAATCA
GCTTATTTGGGATAACCACTAGTCCCATTCAGATATATACAACATTGGCATGCTTCATGA
CAATTGACACATAGATGCCTGGCTATTTGAGGCCTCTCTTTGTGTACATCAATAAAATTG
ATTTTATCTTTGATTTAGGTTTTTTTGTTTGTTTGTGGAACAAGGCGCATAAAAGTTCTT
CTTTCTAGATTTTTTGTGTTCATCACGTTTGTAACCAGA
   
Sequence Name 78-K100834-0022-192-F07C-KT57
GenBank Accession LN513647 [GenBank]
Sequencing primer gene specific primer, KT57: 5'-ATCTGGTTACAAACGTGATGAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K100834-0022-192-F07C-KT57
CTTTATGCGCCTTGTTCCCAACAACAAAAAAACCTAAATCAAAGATAAAATCAATTTTAT
TGATGTACACAAAGAGAGGCCTCAAATAGCCAGGCATCTATGTGTCAATTGTCATGAAGC
ATGCCAATGTTGTATATATCTGAATGGGACTAGTGGTTATCCCAAATAAGCTGATTTTCC
CCACGTGGACTCCTCCCTGGCTGCACCGTTGCAATGGCTTGTACACGTCATCTAAATTGA
GATGCAACGTGGCACGTCACTCAAAGAAGCTCTCACTTTTTGACACCTTCTTTATCTTAT
TTGTTTCTGTCGATCTCTACGGTAATTTTCTCGGTCGCCAATAACCAAAACCTTTCCCGG
AAATTTTCGAATACTCTGAAAGGAAAATAGGAAAATGGCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGA

Last Updated on 06.12.2018 16:11