Sequences from confirmation amplicon


Confirmation sequences for line 193B03 and hit At4g01720 are shown.
Line ID 193B03
Locus At4g01720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:745726
Primers used for PCR IS13 - 8474, show primer details
   
Sequence Name 01-K016447-022-193-B03C-8409
GenBank Accession LN486368 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K016447-022-193-B03C-8409
TGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAATCTACATTTGG
TAGTCCCAATATGCATTTTTGAAGCCAATTTGAAGTTGGAGCTAGAGAGGCTTCACGAGG
AGAATCACAAACTGAAGCATTTATTAGATGAGGTCAGTGAGAGTTACAACGACCTCCAAA
GAAGAGTTTTGTTAGCAAGACAAACACAAGTGGAAGGTCTTCATCATAAACAACATGAGG
TACTTGATAAAATCATAAAAATGATAATAAAATATATATCAACATTTCGGTTTATTAAAA
AATAAATTGTATATTTGTCTTTTTTATGTGGCACCGAGATTAGCTTAGGTTTTAGTGTTC
GATTTATCTGGTTTTGAAAATTTAAAGTGATCTATTAGTTTTGTTTTTCTCGAAAGCAAT
TAAAATTTTAAAACTATGTGTAATATGCGTAAATAATGGATGTAGTTTAGTTATGTAATG
AATTCATAATTTTCATCTTAAATGCCCAGATTGAAAGTCATAAGACGAATAAATACGTGG
CGC
   
Sequence Name 05-K016447-022-193-B03C-IS13
GenBank Accession LN488141 [GenBank]
Sequencing primer gene specific primer, IS13: 5'-AAAGCTGCACGTATTTATTCGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K016447-022-193-B03C-is13
AATCTGGGCATTTAAGATGAAAATTATGAATTCATTACATAACTAAACTACATCCATTAT
TTACGCATATTACACATAGTTTTAAAATTTTAATTGCTTTCGAGAAAAACAAAACTAATA
GATCACTTTAAATTTTCAAAACCAGATAAATCGAACACTAAAACCTAAGCTAATCTCGGT
GCCACATAAAAAAGACAAATATACAATTTATTTTTTAATAAACCGAAATGTTGATATATA
TTTTATTATCATTTTTATGATTTTATCAAGTACCTCATGTTGTTTATGATGAAGACCTTC
CACTTGTGTTTGTCTTGCTAACAAAACTCTTCTTTGGAGGTCGTTGTAACTCTCACTGAC
CTCATCTAATAAATGCTTCAGTTTGTGATTCTCCTCGTGAAGCCTCTCTAGCTCCAACTT
CAAATTGGCTTCAAAAATGCATATTGGGACTACCAAATGTAGATTTCAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATT
TATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC

Last Updated on 06.12.2018 16:11