Sequences from confirmation amplicon


Confirmation sequences for line 198A02 and hit At1g56690 are shown.
Line ID 198A02
Locus At1g56690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21253834
Primers used for PCR BL86 - 8474, show primer details
   
Sequence Name 52-K105140-0022-198-A02C-BL86
GenBank Accession LN507813 [GenBank]
Sequencing primer gene specific primer, BL86: 5'-GTCGTTGTTGTTGTGATGATCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K105140-0022-198-A02C-BL86
ATTTATTTTTTTCTTTAAAGACATAGTACGACGCCATTAAAAGATTCTCCATATTTGATC
TACATTAAAGACGAATCAGTAACCGGTTAATTAAATTGATCCAATTTATTAACTTGATTT
TTCCATTAAAGCTGAATAAATAATTTCAATTATTATAAATTCTAAAAACCCTTATACAAA
CGCTCTGCCCAAAATTGTCAGCTTTAGAGCTTTCGCGCCTCATTTTATGTCATGATTATT
ATCTTTAAACCACATTTGAATCACTCTCATTAGAATCAGTTTGATTAATGAAGCGTTTAA
AGCTAATAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
G
   
Sequence Name 36-K105141-0022-198-A02C-8409
GenBank Accession LN502219 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K105141-0022-198-A02C-8409
TAGCTTTAACGCTTCATTAATCAAACTGATTCTAATGAGAGTGATTCAAATGTGGTTTAA
AGATAATAATCATGACATAAAATGAGGCGCGAAAGCTCTAAAGCTGACAATTTTGGGCAG
AGCGTTTGTATAAGGGTTTTTAGAATTTATAATAATTGAAATTATTTATTCAGCTTTAAT
GGAAAAATCAAGTTAATAAATTGGATCAATTTAATTAACCGGTTACTGATTCGTCTTTAA
TGTAGATCAAATATGGAGAATCTTTTAATGGCGTCGTACTATGTCTTTAAATGAAAAAAA
TAAAATAAAATTCGAATTGTTTTGATTTTTCAGAGATCATC
Primers used for PCR BL85 - 8474, show primer details
   
Sequence Name 53-K105140-0022-198-A02C-BL85
GenBank Accession LN508150 [GenBank]
Sequencing primer gene specific primer, BL85: 5'-AACCATACCTTCTTGCATGTAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K105140-0022-198-A02C-BL85
ACATTCCTCTCGGCATTAGCTCAACACATTCCTAGCTTCTACAATCATCCTATTCTTAAT
ATACCCTGAAACCAACCCGTTCCAGGAAACTACATTTCTCTCAGACATTTCGTCGAACAG
TTGGCGTGCTTCTTTCGGTAACCCGTTTGAGAAATACCCAGATACAATTGAGTTCCATGA
ACCGATTGCTTTAAACTGTAACGAATCGAAAAACTTTCGTGCTTCATTGATTTTCCCGAT
TCGTGAGAGACGTGAGATCTCAAAACTGCAATTCACTCAACGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 37-K105141-0022-198-A02C-OriL
GenBank Accession LN502610 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K105141-0022-198-A02C-OriL
CGTTGAGTGATTGCAGTTTTGAGATCTCACGTCTCTCACGAATCGGGAAAATCAATGAAG
CACGAAAGTTTTTCGATTCGTTACAGTTTAAAGCAATCGGTTCATGGAACTCAATTGTAT
CTGGGTATTTCTCAAACGGGTTACCGAAAGAAGCACGCCAACTGTTCGACGAAATGTCTG
AGAGAAATGTAGTTTCCTGGAACGGGTTGGTTTCAGGGTATATTAAGAATAGGATGATTG
TAGAAGCTAGGAATGTGTTTGAGCTAATGCCTGAGAGGAATGTTGTGTCGTGGACCGCGA
TGGTTAAAGGTTACATGCAAGAGGGTATGGT

Last Updated on 06.12.2018 16:11