Sequences from confirmation amplicon


Confirmation sequences for line 199F09 and hit At3g09090 are shown.
Line ID 199F09
Locus At3g09090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2787964
Primers used for PCR FM93 - 3144, show primer details
   
Sequence Name 52-K031939-022-199-F09C-FM93
GenBank Accession LN507711 [GenBank]
Sequencing primer gene specific primer, FM93: 5'-TGCATCTCAATACAGATCGAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K031939-022-199-F09C-FM93
CGTCATCGGTGGCTTTCGCTCTCTGAACTTATTTTCTCCATAGGAGAGATTCGTTAAGGA
AAGACAGAGCAAACAAACCAGCAGACACTGCCTCGCTCGAGATTTCATATGATCACACTC
TGCGAATACTAGGCAAATTCCCTTGACATAAAATTAAAATCGAATCAGAATCGAAGAACT
GTATCCAACTACGTCGATAGATTTTGTAAAACCTCCCCAAAATCGGGGTTTAGGGAATTT
GGAATGGTGAATAAAAAGGAGAGATCGAGAAGGAGAATGAGACGAAGCTGAGACTCTGTG
TGTGTGGGAGATCGCAGCCGGCGAAATCGAATGACGCTGTGAAGAGTAAACTGCTTGCCG
TTTTGATCTAAGTAAAACGCACGACACAGCTCGCTGTTTAGTTACAAATATGAGAAACGT
CAATTATTCCCTATCACTTCGAATGATCGAAGGAAGATTTTTAACCTTTTCCGTTCTTGA
TTTTTCTATGTAAAATGCGATGAAAAGATTTCAAATCAAACACTGATAGTTTAAACCGAA
GGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCC
TTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGT
GGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCAC
GATGCTCCTCGTGGGTGG
   
Sequence Name 51-K031939-022-199-F09C-3144
GenBank Accession LN507399 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K031939-022-199-F09C-3144
CACTTCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGG
GTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGATTTG
AAATCTTTTCATCGCATTTTACATAGAAAAATCAAGAACGGAAAAGGTTAAAAATCTTCC
TTCGATCATTCGAAGTGATAGGGAATAATTGACGTTTCTCATATTTGTAACTAAACAGCG
AGCTGTGTCGTGCGTTTTACTTAGATCAAAACGGCAAGCAGTTTACTCTTCACAGCGTCA
TTCGATTTCGCCGGCTGCGATCTCCCACACACACAGAGTCTCAGCTTCGTCTCATTCTCC
TTCTCGATCTCTCCTTTTTATTCACCATTCCAAATTCCCTAAACCCCGATTTTGGGGAGG
TTTTACAAAATCTATCGACGTAGTTGGATACAGTTCTTCGATTCTGATTCGATTTTAATT
TTATGTCAAGGGAATTTGCCTAGTATTCGCAGAGTGTGATCATATGAAATCTCGAGCGAG
GCAGTGTCTGCTGGTTTGTTTGCTCTGTCTTTCCTTAACGAATCTCTCCTATGGAGAAAA
TAAGTTCAGAGAGCGTAAAGCCACCGATGACGAGCTGGGCTACCCCGATATGTAAGTTTT
CGATCTGTATGGAAGATGC

Last Updated on 06.12.2018 16:11