Sequences from confirmation amplicon


Confirmation sequences for line 202B02 and hit F20C19 are shown.
Line ID 202B02
Locus F20C19 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9666324
Primers used for PCR CL70 - 8474, show primer details
   
Sequence Name 81-K033216-022-202-B02C-8409
GenBank Accession LN514013 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K033216-022-202-B02C-8409
TGAGCCATTTACAATTGAATATATCGGCATGAAGCCATGTTTTCGGATAAGAGTCCAACG
TTAAGAATCTTTTTGCTGTACATGTTACTCTAATGGGCTATTGTTGGGCTCCAATTTTCT
TGCTGCACAATCGTTGTAGGCTTTAAGTCTTGTATCTTTTGTTTAATAAAATTCACTTGG
GAAAAAAAAAAAAAGTCCCTCCCTTCTACAGCGCGGGAGGGCTTGTTTCCCCCCCCATCT
CTCCTTCTTTGAAAAGCATGCCCCAAAAAAAAAAAAGGGTTTCAAAAAAAAAAACCCCCA
AAAATTTTCATTTTTTCCCCAAAAGAAAAAAACTTTTTTGGAAGGTTTTCAGGGTTTCCC
CGAACCCCAAAATCAAAAATTTTTCCCCAATTTAATTTTCCATTTTCAAATCGAAAAAAA
TTATCCAATTTCAAAATTTTAAAATTTGTTTTCAAAACCTGGGGGGGAAATTGAAATTGA
ACTTGGGCCTAAAAAAAAAAACCGGCCCAAAAAAAAAAAACCGGGGAAACCTACTGCTCC
AACGGGGAAAACAAAAATTTTTCCAACCCAAAAAAAGCCCCCCCCCCCCCCCCCAACAAG
GGTAAATTCTAGAAAAAAAAAAAAAAAAATTTTTTCGAATTTTCCAACGGGGGCTTAAAC
ACCCCCGGGAGGAAA
   
Sequence Name 84-K033216-022-202-B02C-CL70
GenBank Accession LN514375 [GenBank]
Sequencing primer gene specific primer, CL70: 5'-CAATCCAACAGAGTTTCTACCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K033216-022-202-B02C-CL70
GAAAACTCATCTTCTTCATCTTCCTAGACTTACCACTAGTCGCAGTCGCAGCAGCAGCCT
CTCTCAGGCTAGGAAAACTCTGTGTATCCGCCGTCGCAGCAGTAGCTTCCGCCGCTTGTT
CTTCATCGGCACGCTCTGCTTCATCAGCCCAAGCTCCAATTCCAATTCCACCCCAAGCTT
TCGACATCAATTTTGAAATTTCAGAATTTGACTAATTCTCTCAGATCTGAAATCGCAAAT
TCAAATCGGGAAAAAGTATTAGATCTAGGGTTCCGATGAAACCTTGAAACCGTCACAGAA
ATGTTTATTCCTTTTGCGTAAAGATTGAAATTTTTTGGGATTTATTCTCTTTGAAGCCTT
TTTTTTTTTTGGGGGCAGGCTTCCCAACAAAGGAAAAATGGGGGGAAAAACAACCCATCC
CCCCCTGAAAAACCGAGGGACTTTTTTTTTTTTCCCCAGGGGATTTTTTTAAAAAAAAAA
AACCAAAATTTAAGCCCCCCACCGTTGGGGCCCAAAAAAAATGGGCCCCCAAAAAACCCC
CTTTAAAAAAACTGGTCCCCAAAAAAAATTTTAAAGTTGGGACTTTTACCAAAAAAAGGG
GTTCCCGGCCAAAATTTCAATTTGAAAAGGGTTTCTGGCCGGGAAAACCCCCGGGGACCC
CCAAGGATTAGAGGGGTCAATTGGGAAAAAAAAAAAAAGAATTTCCCATTTTTTTTCCAA
TCAAAAAAGGAAAATCCCCCCCCCTTTTTTAAAAAAGAAAATTCCTTTTTAAAAAAACAA
AAAATAAAAACCCG

Last Updated on 06.12.2018 16:11