Sequences from confirmation amplicon


Confirmation sequences for line 203G10 and hit At3g56400 are shown.
Line ID 203G10
Locus At3g56400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20909730
Primers used for PCR IS14 - 8474, show primer details
   
Sequence Name 07-K016199-022-203-G10C-8409
GenBank Accession LN489036 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K016199-022-203-G10C-8409
GTATATTTCCCGGACATGAAGCCATTTACAATTGTTCACTTCGGACCTTGGGAATTTGGC
ATTAAGAATCTCCTTTTGTCCATATTTCCTCCAAGAAAATGCGTCCTCAAGTATAGTCGA
CTCTATAGTACACGTCTCCGATCTCTTTCTGCTCATAATATTAATGAAATCAATTAATCA
TTTGGCTACTTATGATTCTTGCAACCAAAGCTAAATGTCTTGTTTGTTTGTGACTAACCA
TAACAGTAAGTAAATCCTAGATGATAGTTTTTTCCATGAGTATGGATGATCACTAGATAT
ATGGTACATATGATCAACGTAATCGGTCTAATAGAACTACGATGGATTGATTTTATATCA
TTATGGTTAATAATTGAGTAAGAGAGAGAAAAGGGCGAAA
   
Sequence Name 08-K016199-022-203-G10C-IS14
GenBank Accession LN489501 [GenBank]
Sequencing primer gene specific primer, IS14: 5'-AAGAAAGTAAGTTTCGCCCTTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K016199-022-203-G10C-is14
CTCAATTATTAACCATAATGATATAAAATCAATCCATCGTAGTTCTATTAGACCGATTAC
GTTGATCATATGTACCATATATCTAGTGATCATCCATACTCATGGAAAAAACTATCATCT
AGGATTTACTTACTGTTATGGTTAGTCACAAACAAACAAGACATTTAGCTTTGGTTGCAA
GAATCATAAGTAGCCAAATGATTAATTGATTTCATTAATATTATGAGCAGAAAGAGATCG
GAGACGTGTACTATAGAGTCGACTATACTTGAGGACGCATTTTCTTGGAGGAAATATGGA
CAAAAGGAGATTCTTAATGCCAAATTCCCAAGGTCCGAAGTGAACAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
Primers used for PCR CT42 - 3144, show primer details
   
Sequence Name 18-K105032-0022-203-G10C-CT42
GenBank Accession LN494299 [GenBank]
Sequencing primer gene specific primer, CT42: 5'-TAGGACTCTTGTGATCTTCGGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K105032-0022-203-G10C-CT42
GTCTTGCTCTTGGGAGTTCTGCGTTGGTGTTACACGTGTGGTTTCCTATGTATGTGATAC
TGAACATCTTGGGTTCGAGCTCAACCTTCTGGACTTGCTTTGTTGCCTTGCACCCTTGGG
TATACTTGTGTGTGCATCTAAAGTAACTTCTGCACAACATAAATTAAAATAAAATAATTT
AGGCCAAAATATATATGCCTCAAGCCCACAACCAATTAAGCTCAGACCACATTTATGGTA
GATATAATTGCGGTCCATGTGTAATAATACCCGGCAACATGTCACATATCAGATACGAAT
ATATATAGACAAAACTATATAGGGGTAAAAAGGACCTTGGGAATTTGGCATTAAGAATCT
CCTTTTGTCCATATTTCCTCCAAGAAAATGCGTCCTCAAGTATAGTCGACTCTATAGTAC
ACGTCTCCGATCTCTTTCTGCTCATAATATTAATGAAATCAATTAATCATTTGGCTACTT
ATGATTCTTGCAACCAAAGCTAAATGTCTTGTTTGTTTGTGACTAACCATAACAGTAAGT
AAATCCTAGATGATAGTTTTTTCCATGAGTATGGATGATCACTAGATATATGGTACATAT
GATCAACGTAATCGGTCTAATAGAACTACGATGGATTGATTTTATATCATTATGGTTAAT
AATTGAGTAAGAGAGAGAAAAGGGCGAAACTTACTTTCTTTTGTAGCATCCTCTTTTACC
CTTAACGGGTCCCAATCTTTTCCGACTATCGCCGGAATCTTCAAACTTGCCGTCGTTATC
ACATGAAGCATTTTGAGATCCCTCAACGGCGGCGAGGGATGAGGAGGAGGAGATGGGTTC
GAAGGTATCAAGAACGGAGATGGTGTTATTGAAACACACTAAGATTTTAGCCACTAGATC
CTCTAGACCGGACCCGGGTTGAGAGAGGAGTTGCTGAAGCTGAGTTGTTAGTCATGGCCT
TCAACGAGTTGGTTCATAACTTTA
   
Sequence Name 34-K105033-0022-203-G10C-3144
GenBank Accession LN501410 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K105033-0022-203-G10C-3144
CCTTGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGGATATCAAAAT
TATCTTCATAGAAAAACGGATCATATCATTTGGATATCAAATTTAACTCGAGATTTCAAA
TAAATAATTCACTATTCCAACTGATCTTACTACATCAGTAAAAAAAAATTGGTAGTACAA
TCTCGAGGAACAAAAAAGTTTACAAATGAAATTTAGAATCTTGTTTAATCTTTAAATGGA
TAGTTTTTCATAACTCTTTTAATTAGTCTAGTTTTTGATACAAATTTTATCCGGTTATAG
AAAACATTAAAGGACCCTAAGTTTGGATTTCAGCAAAATTATATATATTCCATTCTCACT
TAGGGATGAAATGACATCATTCATCACTACGTCATTTGAGCTTACGTCAAGCTCACCATA
AGCAAAAGAAATGGGTGGATAAGTTCAAGTTCCATCTCATTTCCTCACACATCTCTCTTT
CTTCCTTCTCATTTCCTCGCATCTTCCTCTAAAAAAAAAAATCAAAGAAACCAACAAAAT
AAAAAGTGCCCATCATTACAAATCTTATTTCCACCTTATATTTACACACACTTCTCTTCT
TTCCTTTAATCATACAAACCACCCCAAGAGGAAAGAAAAATAAGATACCACTCACCAAAA
ACTTCCTCAAAACTAACAAATGGATACTAATAAAGCAAAAAAGCTTAAAGTTATGAACCA
ACTCGTTGAAGGCCATGACTTAACAACTCAGCTTCAGCAACTCCTCTCTCAACCCGGGTC
CGGTCTAGAGGATCTAGTGG
Primers used for PCR 59wf - 8474, show primer details
   
Sequence Name 20-K118238-0022-203-G10C-59wf
GenBank Accession LN495410 [GenBank]
Sequencing primer gene specific primer, 59wf: 5'-TCCCAACTCCCAATTATATCTGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K118238-0022-203-G10C-59wf
GGCATTATTTCGGACTGTTGATTGTAATTTCGTGTCTCGTTATCTATTTGAAATAGTAGG
TCTATTTAGGGGGTATACATCTGGATTGATAGCTGATAAGGACTATATACATGTCTTTTT
GAATTTTTGATATATAAACTACTATTCAAAATTATCTTCATAGAAAAACGGATCATATCA
TTTGGATATCAAATTTAACTCGAGATTTCAAATAAATAATTCACTATTCCAACTGATCTT
ACTACATCAGTAAAAAAAAATTGGTAGTACAATCTCGAGGAACAAAAAAGTTTACAAATG
AAATTTAGAATCTTGTTTAATCTTTAAATGGATAGTTTTTCATAACTCTTTTAATTAGTC
TAGTTTTTGATACAAATTTTATCCGGTTATAGAAAACATTAAAGGACCCTAAGTTTGGAT
TTCAGCAAAATTATATATATTCCATTCTCACTTAGGGATGAAATGACATCATTCATCACT
ACGTCATTTGAGCTTACGTCAAGCTCACCATAAGCAAAAGAAATGGGTGGATAAGTTCAA
GTTCCATCTCATTTCCTCACACATCTCTCTTTCTTCCTTCTCATTTCCTCGCATCTTCCT
CTAAAAAAAAAAATCAAAGAAACCAACAAAATAAAAAGTGCCCATCATTACAAATCTTAT
TTCCACCTTATATTTACACACACTTCTCTTCTTTCCTTTAATCATACAAACCACACCAAG
AGGAAAGAAAAATAAGATCCCCCTCACCAAAAACTTCCTCAAAACTAACAAATGGATACT
AATAAAGCAAAA
   
Sequence Name 06-K118237-0022-203-G10C-8409
GenBank Accession LN489004 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K118237-0022-203-G10C-8409
TCATTGTTCCTTCGGACCTTGGGAATTTGGCATTAAGAATCTCCTTTTGTCCATATTTCC
TCCAAGAAAATGCGTCCTCAAGTATAGTCGACTCTATAGTACACGTCTCCGATCTCTTTC
TGCTCATAATATTAATGAAATCAATTAATCATTTGGCTACTTATGATTCTTGCAACCAAA
GCTAAATGTCTTGTTTGTTTGTGACTAACCATAACAGTAAGTAAATCCTAGATGATAGTT
TTTTCCATGAGTATGGATGATCACTAGATATATGGTACATATGATCAACGTAATCGGTCT
AATAGAACTACGATGGATTGATTTTATATCATTATGGTTAATAATTGAGTAAGAGAGAGA
AAAGGGCGAAACTTACTTTCTTTTGTAGCATCCTCTTTTACCCTTAACGGGTCCCAATCT
TTTCCGACTATCGCCGGAATCTTCAAACTTGCCGTCGTTATCACATGAAGCATTTTGAGA
TCCCTCAACGGCGGCGAGGGATGAGGAGGAGGAGATGGGTTCGAAGGTATCAAGAACGGA
GATGGTGTTATTGAAACACACTAAGATTTTAGCCACTAGATCCTCTAGACCGGACCCGGG
TTGAGAGAGGAGTTGCTGAAGCTGAGTTGTTAAGTCATGGCCTTCAACGAGTTGGTTCAT
AACTTTAAGCTTTTTTGCTTTATTAGTATCCATTTGTTAGTTTTGAGGAAGTTTTTGGTG
AGTGGTATCTTATTTTTCTTTCCTCTTGGTGTGGTTTGTATGATTAAAGGAAAGAAGAGA
AGTGTGTGTAAATATAAGGTGGAAATAAGATTTGTAATGATGGGCACTTTTTATTTTGTT
GGTTTCTTTGATTTTTTTTTTTAAAGAAAATTCCAGGAAATTAGAAGGAAAA

Last Updated on 06.12.2018 16:11