Sequences from confirmation amplicon


Confirmation sequences for line 207G10 and hit At3g53130 are shown.
Line ID 207G10
Locus At3g53130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19695251
Primers used for PCR MS56 - 8474, show primer details
   
Sequence Name 02-K017630-022-207-G10C-MS56
GenBank Accession LN486826 [GenBank]
Sequencing primer gene specific primer, MS56: 5'-ACGTGAAAGTGAAACTACACATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K017630-022-207-G10C-ms56
AACCCACTAGTATTGGTTTTATATGGTTTTATATGGTTTTTAAGGTTAATGTTACGTGTG
GTCCATATGGAATGGGATGGTTTTAGACCTTAGTCTATTATTTAGAAATTCAAAAAAAAA
TAAAATTTTGATTTTCGTAATTACTGAAAAATGGAGAACAAACACAAGCTGTATGAAAGT
TTGATTTGAAATGATAACACAATTACCAAACTTTAATGGTACTAGCATCTCACTGTTCAA
AATTCACATTTCCGATGGTACAACTATTTTCCATAATTCATTTTTTTGTGGTTATTTCTC
AAGAGTACTATGAAACAAGTCCATTCACACCAATCAATTATCATGAAAACATAAATTCTG
GTTTTTACCTTTGGCTCACCTTCATATACAATTGGTTTCCAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA
   
Sequence Name 06-K017630-022-207-G10C-8409
GenBank Accession LN488592 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K017630-022-207-G10C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGAAACCAATTGT
ATATGAAGGTGAGCCAAAGGTAAAAACCAGAATTTATGTTTTCATGATAATTGATTGGTG
TGAATGGACTTGTTTCATAGTACTCTTGAGAAATAACCACAAAAAAATGAATTATGGAAA
ATAGTTGTACCATCGGAAATGTGAATTTTGAACAGTGAGATGCTAGTACCATTAAAGTTT
GGTAATTGTGTTATCATTTCAAATCAAACTTTCATACAGCTTGTGTTTGTTCTCCATTTT
TCAGTAATTACGAAAATCAAAATTTTATTTTTTTTTGAATTTCTAAATAATAGACTAAGG
TCTAAAACCATCCCATTCCATATGGACCACACGTAACATTAACCTTAAAAACCATATAAA
ACCATATAAAACCAATACTAGTGGGTTCTATAGCGATGTGTAGTT

Last Updated on 06.12.2018 16:11