Sequences from confirmation amplicon


Confirmation sequences for line 208B08 and hit At5g03720 are shown.
Line ID 208B08
Locus At5g03720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:973355
Primers used for PCR KN14 - 8474, show primer details
   
Sequence Name 57-K103246-0022-208-B08C-KN14
GenBank Accession LN509386 [GenBank]
Sequencing primer gene specific primer, KN14: 5'-CTCCATTCAAACGAAACCATAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K103246-0022-208-B08C-KN14
ATGAGAGTTATTATAGATCCAATCGAGGCCAAAATTTCATAGATACAAAACTCAGTGTTA
CATGATAGAGAGATTAAACAAATCCCAAAAAGAAAAGAAAATTACAGGTAATTTTTTACA
AGTTATCAAAAAACGAGTTTCAAGAATCTATACAAAATGGTTTTGGTCATCAAAAAGATT
ACAGAAACATAAAAACAAAACAAAACTGAAAGAAGAAGAAGAAGAAGAAGAATGAGTCTA
TCTNGAAAACAGAAACAGAGCANGAGAGATGATTACATAAGTGTTAAGCTTCGTCAGTAT
TTACCAATTGTAACAGAAACAGAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCANAAATGTAGATTTCCGCA
   
Sequence Name 09-K103247-0022-208-B08C-8409
GenBank Accession LN490136 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K103247-0022-208-B08C-8409
GAGCCATTTACATTGAATATATCCTGCTCTGTTTCTGTTACAATTGGTAAATACTGACGA
AGCTTAACACTTATGTAATCATCTCTCTTGCTCTGTTTCTGTTTTCTAGATAGACTCATT
CTTCTTCTTCTTCTTCTTCTTTCAGTTTTGTTTTGTTTTTATGTTTCTGTAATCTTTTTG
ATGACCAAAACCATTTTGTATAGATTCTTGAAACTCGTTTTTTGATAACTTGTAAAAAAT
TACCTGTAATTTTCTTTTCTTTTTGGGATTTGTTTAATCTCTCTATCAGGTAACACTGAG
TTTTGTATCTATGAAATTTTGGCCTCGATTGGATCTTATAATAACTTCTCATCTGAAATA
AGCTTGTAATCTTGTATACTTATGGTTTCGTTAGGAATGGA

Last Updated on 06.12.2018 16:11