Sequences from confirmation amplicon


Confirmation sequences for line 208H06 and hit At2g16280 are shown.
Line ID 208H06
Locus At2g16280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7050966
Primers used for PCR AY10 - 8474, show primer details
   
Sequence Name 29-K016210-022-208-H06C-8409
GenBank Accession LN499072 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K016210-022-208-H06C-8409
ATTTCCGGACATGAAGCCAAATAATAACAAAACAAAAACACTTTTGGTTTTTTCTTCTTC
TTCTCTCTAAATCATAAAAACACAAGTTTTCGACAGATCAACTGTTTTCTTCATTACGTG
TTCTTCAATGGCGTCACTTCACTGATTCTATTCCTATCAAACCCACTTACCCATTCCCTT
TTATATGCACAAATTCTCATAAAGTTTTCAATTTTATTCCATTTTTCTCGGAAGCCATGG
AAGCTGCTAATGAGCCTGTTAATGGCGGATCCGTACAGATCCGAACAGAGAACAACGAAA
GACGAAAGCTTCCTAATTTCTTACAAAGCGTCAACATGAAATACGTCAAGCTAGGTTATC
ATTACCTCATTACTCATCTCTTCAAGCTCTGTTTGGTTCCATTAATGGCGGTTTTAGTCA
CAGAGATCTCTCGATTAACAACAGACGATCTTTACCAGATTTGGCTTCATCTCCAATACA
ATCTCGTTGCTTTCATCTTTCTCTCTGCTTTAGCTATCTTTGGCTCCACCGTTTACATCA
TGAGTCGTCCCAGATCTGTTTATCTCGTTGATTACTCTTGTTATCTTCCTCCGGAGAGTC
TTCAGGTTAAGTATCAGAAGTTTATGGATCATTCTAAGTTGATTGAAGATTTCAATGAGT
CATCTTTAGAGTTTCAGAGGAAGATTCTTGAACGTTCTGGTTTAAGAGAAGAGACTTATC
TCCCTGAAGCTTTACATTGTATCCC
   
Sequence Name 30-K016210-022-208-H06C-AY10
GenBank Accession LN499508 [GenBank]
Sequencing primer gene specific primer, AY10: 5'-AGCTTATCAAGAGCACCAAACATT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K016210-022-208-H06C-ay10
CTCAGATTCCTCACGAGCCGCCATCATCGTAGGCCTCGGAGGGATACAATGTAAAGCTTC
AGGGAGATAAGTCTCTTCTCCTAAACCAGAACGTTCAAGAATCTTCCTCTGAAACTCTAA
AGATGACTCATTGAAATCTTCAATCAACTTAGAATGATCCATAAACTTCTGATACTTAAC
CTGAAGACTCTCCGGAGGAAGATAACAAGAGTAATCAACGAGATAAACAGATCTGGGACG
ACTCATGATGTAAACGGTGGAGCCAAAGATAGCTAAAGCAGAGAGAAAGATGAAAGCAAC
GAGATTGTATTGGAGATGAAGCCAAATCTGGTAAAGATCGTCTGTTGTTAATCGAGAGAT
CTCTGTGACTAAAACCGCCATTAATGGAACCAAACAGAGCTTGAAGAGATGAGTAATGAG
GTAATGATAACCTAGCTTGACGTATTTCATGTTGACGCTTTGTAAGAAATTAGGAAGCTT
TCGTCTTTCGTTGTTCTCTGTTCGGATCTGTACGGATCCGCCATTAACAGGCTCATTAGC
AGCTTCCATGGCTTCCGAGAAAAATGGAATAAAATTGAAAACTTTATGAGAATTTGTGCA
TATAAAAGGGAATGGGTAAGTGGGTTTGATAGGAATAGAATCAGTGAAGTGACGCCATTG
AAGAACACGTAATGAAGAAAACAGTTGATCTGTCGAAAACTTGTGTTTTTATGATTTAGA
GAGAAGAAGAAGAA

Last Updated on 06.12.2018 16:11